Cytotoxic T lymphocyte
View Patent ↗The present invention relates to a T lymphocyte having an activity to induce a T lymphocyte recognizing an antigen and a technique to use the T lymphocyte.
1. A method for inducing a T lymphocyte, comprising:
transducing a T lymphocyte with an RNA encoding an antigen of interest, wherein said T lymphocyte comprising the transduced RNA functions as an antigen-presenting cell;
treating the T lymphocyte transduced with the RNA by gamma ray irradiation or with mitomycin; and
stimulating a T lymphocyte with the T-lymphocyte transduced with the RNA obtained in the above step, thereby inducing the T lymphocyte which recognizes the antigen.
2. The method according to claim 1 , wherein the T lymphocyte transduced with the RNA is a CD4-positive cell.
3. The method according to claim 1 , wherein the T lymphocyte which recognizes the antigen is a cytotoxic T lymphocyte positive to CD8.
4. The method according to claims 1 , wherein the antigen is a tumor-associated antigen or an antigen of an infectious microorganism.
5. The method according to claim 4 , wherein the RNA is an RNA prepared from a cancer tissue.
6. The method according to claim 4 , wherein the antigen is EBNA3A and/or CMVpp65.
7. The method according to claim 1 , wherein the T lymphocyte transduced with the RNA is activated with phytohemagglutinin.
8. The method according to claim 1 , which further comprises the process of proliferating the induced T lymphocyte.
9. The method of claim 1 , wherein the antigen of interest is selected from the group consisting of MAGE-A4, SAGE, LAGE, WT-1, and hTERT.
10. The method of claim 1 , wherein the antigen of interest is MAGE-A4.
11. The method of claim 1 , wherein the antigen of interest is SAGE.