Dyes for Membranes and Biological Structures
The present invention relates to the use of one or more natural dyes, alone or in combination with other dyes for preparation of a composition to stain membranes and biological structures, in order to facilitate their identification during surgical procedures. The present invention relates to the use of one or more natural dyes, alone or in combination with other dyes, to stain membranes and biological structures, in order to facilitate their identification during surgical procedures.
1 . A method of treating biological tissues, membranes or other structures, comprising selecting a natural dye selected from the group consisting of lutein, zeaxanthin, urucum, lycopene, astaxanthin, beta-carotene, and beta-cryptoxanthin, alone or in combination with each other or other synthetic dyes, and applying the dye to the biological tissue, membranes or other structure.
2 . A method as defined in claim 1 , wherein applying the dye to the biological tissues, membranes or other structures, results in staining of the biological tissue, membranes or other structure.
3 . A method as defined in claim 2 , further comprising the step of separating the stained biological tissues, membranes or other structures from surrounding tissue.
4 . A method as defined in claim 3 , wherein the separation step is selected from the group consisting of ophthalmic, brain, abdominal, arthro-orthopedic, plastic, gynecological and cancer surgeries.
5 . A method as defined in claim 1 , further comprising the step of adding a second dye selected from the group consisting of brilliant blue, indocyanine green, infracyanine green, trypan blue, patent blue, bromophenol blue, fast green, indigo carmine, evans blue, light green, triamcinolone, crystal violet, fluorescein, fluormetolone acetate and congo red and combinations thereof.
6 . A method as defined in claim 1 , wherein said dye is applied to the biological tissue, membranes or other structure to protect it from damage by light when exposed during a medical procedure.
7 . A method as defined in claim 6 , wherein said dye consists essentially of lutein and zeaxanthin.
8 . A method as defined in claim 1 , wherein the biological tissue, membranes or other structure consists of biological tissue, membranes or other structure of the eye and said dye is applied by injecting it onto the biological tissue, membranes or other structure of the eye.
9 . A method as defined in claim 8 , wherein an ocular implant including lutein and zeaxanthin is inserted in the eye tissue (external surface or intraocularly) for protection against macular degeneration or to delay the progression of macular degeneration.
10 . A method as defined in claim 8 , wherein the dye comprises a slow-release form of a combination consisting essentially of lutein and zeaxanthin placed in the eye.
11 . A composition for staining biological tissues, membranes or other structures, comprising a natural dye selected from the group consisting of lutein, zeaxanthin, urucum, lycopene, astaxanthin, beta-carotene, and beta-cryptoxanthin, alone or in combination with each other and a pharmaceutically acceptable vehicle.
12 . A composition according to claim 11 , further comprising a dye selected from the group consisting of brilliant blue, indocyanine green, infracyanine green, trypan blue, patent blue, bromophenol blue, fast green, indigo carmine, evans blue, light green, triamcinolone, crystal violet, fluorescein, fluormetolone acetate and congo red and combinations thereof.
13 . A composition according to claim 12 , wherein the dye is present in a concentration of 0.001% to 20%.
14 . A composition according to claim 11 , wherein said natural dye consists essentially of lutein and zeaxanthin, alone or combined.
15 . A composition according to claim 11 , wherein the vehicle is selected from the group consisting of phosphate buffer, balanced salt solution, polyvinyl alcohol, benzyl alcohol, purified water, sodium phosphate, sodium chloride, calcium chloride, magnesium chloride, potassium chloride, sodium citrate, sodium acetate, boric acid, sodium borate, methyl cellulose and its derivatives, hyaluronic acid, dextran, sodium polysorbate, Tweens, chondroitin sulfate, sodium edetate, propylene glycol, polyethylene glycol, phenyl sodium, potassium phthalate, potassium phosphate, citric acid, carbomer 934, Carbopol, metaphosphoric acid, glycocholic acid, acetic acid, and combinations thereof.
16 . A composition according to claim 12 , wherein the composition comprises a first and a second dye at a rate ranging between 0.001% and 20%.
17 . A composition for staining biological tissues, membranes or other structures, comprising (a) one or more dyes selected from the group consisting of those listed in the first three rows of following table within the recited range; (b) optionally one or more dyes selected from the group consisting of those listed in the remaining “Active” rows of the following table within the recited range; and (c) one or more vehicles selected from the group consisting of those listed in the “Vehicle” rows of the following table within the recited range:
Preferable
Function
Concentration
Concentration
within the
Component
[% w/v]
[% w/v]
composition
Lutein
0.001% to 20%
0.01%-5%
Active
Zeaxanthin
0.0007% to 20%
0.007%-0.5%
Active
Urucum
0.01% to 20%
Active
Brilliant Blue
0.01% to 0.05%
0.0125%-0.025%
Active
Green Indocianine
0.001% to 20%
0.5%
Active
Green Infracianine
0.001% to 20%
0.5%
Active
Trypan Blue
0.001% to 20%
0.04%
Active
Patent Blue
0.05% to 0.5%
0.24%
Active
Bromophenol Blue
0.05% to 0.5%
0.2%
Active
Fast green
0.001% to 20%
Active
Indigo Carmine
0.001% to 20%
Active
Evans Blue
0.001% to 20%
Active
Light Green
0.001% to 20%
Active
Triancinolone
0.001% to 20%
Active
Crystal Violet
0.001% to 20%
Active
Fluorescein
0.001% to 20%
Active
Fluormetolone Acetate
0.001% to 20%
Active
Congo Red
0.001% to 20%
Active
Phosphate Buffer
0.001% to 99.99%
Vehicle
Balanced Saline
0.001% to 99.99%
Vehicle
Solution
Polyvynilic Acid
0.001% to 99.99%
Vehicle
Benzylic Acid
0.001% to 99.99%
Vehicle
Purified Water
0.001% to 99.99%
Vehicle
Sodium Phosphate
0.001% to 99.99%
Vehicle
Sodium Cloride
0.001% to 99.99%
Vehicle
Calcium Cloride
0.001% to 99.99%
Vehicle
Magnesium Cloride
0.001% to 99.99%
Vehicle
Potassium Cloride
0.001% to 99.99%
Vehicle
Sodium Citrate
0.001% to 99.99%
Vehicle
Sodium Acetate
0.001% to 99.99%
Vehicle
Boric Acid
0.001% to 99.99%
Vehicle
Sodium Borate
0.001% to 99.99%
Vehicle
Methylcelulose and its
0.001% to 99.99%
Vehicle
derivatives
Hyaluronic Acid
0.001% to 99.99%
Vehicle
Dextrane
0.001% to 99.99%
Vehicle
Sodium Polisorbate
0.001% to 99.99%
Vehicle
Tweens
0.001% to 99.99%
Vehicle
Chondroitin Sulfate
0.001% to 99.99%
Vehicle
Sodium Edetate
0.001% to 99.99%
Vehicle
Propyleno glycol
0.001% to 99.99%
Vehicle
Polyethyleno glycol
0.001% to 99.99%
Vehicle
Sodium
0.001% to 99.99%
Vehicle
Phenylphosphate
Potassium Phthalate
0.001% to 99.99%
Vehicle
Potassium Phosphate
0.001% to 99.99%
Vehicle
Citric Acid
0.001% to 99.99%
Vehicle
Carbomer 934
0.001% to 99.99%
Vehicle
Carbopol
0.001% to 99.99%
Vehicle
Metaphosforic Acid
0.001% to 99.99%
Vehicle
Glycocol
0.001% to 99.99%
Vehicle
Acetic Acid
0.001% to 99.99%
Vehicle