FUNCTIONS AND TARGETS OF LET-7 MICRO RNAS
The present invention concerns methods and compositions for treating or assessing treatment of diseases related to mis-expression of genes or genetic pathways that can be modulated by let-7. Methods may include evaluating patients for genes or genetic pathways modulated by let-7, and/or using an expression profile to assess the condition of a patient or treating the patient with an appropriate miRNA.
1 . A method of modulating gene expression in a lung cancer cell of a subject having or suspected of having lung cancer comprising administering to the cancer cell an amount of an isolated nucleic acid comprising a let-7 nucleic acid sequence in combination with an isolated nucleic acid comprising a miR-34, a miR-124, a miR-126, or miR-147 nucleic acid sequence in an amount sufficient to inhibit proliferation of the lung cancer cell and treat the subject.
2 .- 7 . (canceled)
8 . The method of claim 1 , wherein the let-7 nucleic acid comprises at least one of hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7c, hsa-let-7d, hsa-let-7e, hsa-let-7f-1, hsa-let-7f-2, hsa-let-7g, hsa-let-71, or a segment thereof.
9 . The method of claim 1 , wherein the let-7 nucleic acid is an inhibitor of let-7 function.
10 . (canceled)
11 . (canceled)
12 . The method of claim 1 , wherein the isolated let-7 nucleic acid and the miR-34, the miR-124, the miR-126, or the miR-147 nucleic acids are recombinant nucleic acids.
13 . The method of claim 12 , wherein the recombinant nucleic acid is RNA.
14 . The method of claim 12 , wherein the recombinant nucleic acid is DNA.
15 . The method of claim 14 , wherein the recombinant nucleic acid comprises a let-7 expression cassette.
16 . (canceled)
17 . The method of claim 1 , wherein the let-7 nucleic acid and the miR-34, the miR-124, the miR-126, or the miR-147 nucleic acids are synthetic nucleic acid.
18 .- 34 . (canceled)