SYSTEM AND METHOD FOR REPEAT MEASUREMENT OF SINGLE CELLS USING VIBRATIONAL SPECTROSCOPY
This disclosure concerns a high-throughput cytometry system including a handling system that presents single cells to at least one quantum cascade laser (QCL) source. The QCL laser source is configured to deliver light successively to single cells over a time period in order to make successive measurements of induced resonant mid-infrared absorption by one or more analytes of the cells. The cytometry system also includes a mid-infrared detection facility that detects the successively transmitted mid-infrared wavelength light, wherein the transmitted mid-infrared wavelength light is used to identify a changing cell characteristic over the time period.
1 . A high-throughput cytometry system, comprising:
a handling system that presents single cells to at least one quantum cascade laser (QCL) source;
the at least one QCL source configured to deliver light successively over a time period to a cell within the single cells presented in order to induce resonant mid-infrared absorption by one or more analytes of the cell; and
a mid-infrared detection facility that detects a transmitted mid-infrared wavelength light, wherein the transmitted mid-infrared wavelength light is used to identify a changing cell characteristic over the time period.
2 . The system of claim 1 , further comprising, exposing the cells to a treatment, wherein identifying the cell characteristic is used to determine a response to the treatment.