Single particle QCL-based mid-IR spectroscopy system with analysis of scattering
View Patent ↗This disclosure concerns a system with scattering analysis including a handling system that presents a single particle to at least one quantum cascade laser (QCL) source. The QCL laser source is configured to deliver light to the single particle in order to induce resonant mid-infrared absorption in the particle or an analyte within the particle. A mid-infrared detection facility detects the mid-infrared wavelength light scattered by the single particle, wherein a wavelength and angle analysis of the scattered mid-IR wavelength light is used to determine analyte-specific structural and concentration information.
1. A system with scattering analysis, comprising:
a handling system that presents a single particle, that has not been chemically conditioned for analysis, to at least one quantum cascade laser (QCL) source;
the at least one QCL laser source configured to deliver light to the single particle in order to induce resonant mid-infrared absorption in the particle or an analyte within the particle; and
a mid-infrared detection facility that detects a mid-infrared wavelength light scattered by the single particle,
wherein an analysis of intensity versus at least one of wavelength and angle of the scattered mid-infrared wavelength light is used to determine analyte-specific structural and concentration information.
2. The system of claim 1 , wherein the particle is a cell.
3. The system of claim 1 , further comprising a sort facility for sorting the single particle according to one or more of a transmitted mid-infrared wavelength light and the scattered mid-infrared wavelength light.
4. The system of claim 1 , wherein the analysis further includes an analysis of absorption measurements.
5. The system of claim 1 , wherein the analysis is not dependent on imaging.
6. The system of claim 1 , wherein the at least one QCL source is configured to deliver light to a particle in a volume of a microfluidic handling system.
7. The system of claim 1 wherein the particle is a liquid droplet.
8. The system of claim 1 wherein the particle is measured repeatedly.
9. The system of claim 1 wherein the particle is an embryo and the analysis further provides information related to the viability of the embryo.
10. A system with scattering analysis, comprising:
a handling system that presents a single particle to at least one quantum cascade laser (QCL) source;
the at least one QCL source configured to deliver light to the single particle in order to induce resonant mid-infrared absorption in the particle or an analyte within the particle; and
a mid-infrared detection facility that detects a mid-infrared wavelength light scattered by the single particle,
wherein an analysis of intensity versus at least one of wavelength and angle of the scattered mid-infrared wavelength light is used to determine analyte-specific structural and concentration information, and
wherein the at least one QCL source is configured to deliver light to a particle in a volume of a microfluidic handling system.
11. The system of claim 10 , wherein the particle is a cell.
12. The system of claim 10 , further comprising a sort facility for sorting the single particle according to one or more of a transmitted mid-infrared wavelength light and the scattered mid-infrared wavelength light.
13. The system of claim 10 , wherein the analysis further includes an analysis of absorption measurements.
14. The system of claim 11 , wherein the cell remains viable after the analysis.
15. A system with scattering analysis, comprising:
a handling system that presents a single cell to at least one quantum cascade laser (QCL) source;
the at least one QCL source configured to deliver light to the single cell in order to induce resonant mid-infrared absorption in the particle or an analyte within the cell; and
a mid-infrared detection facility that detects a mid-infrared wavelength light scattered by the single cell,
wherein an analysis of intensity versus at least one of wavelength and angle of the scattered mid-infrared wavelength light is used to determine analyte-specific structural and concentration information, and
wherein the cell remains viable after the analysis.
16. The system of claim 15 , further comprising a sort facility for sorting the single cell according to one or more of a transmitted mid-infrared wavelength light and the scattered mid-infrared wavelength light.
17. The system of claim 15 , wherein the analysis further includes an analysis of absorption measurements.
18. The system of claim 15 , wherein the at least one QCL source is configured to deliver light to the single cell in a volume of a microfluidic handling system.
19. The system of claim 15 wherein the single cell is contained within a liquid droplet.
20. The system of claim 15 wherein the single cell is measured repeatedly.