Methods and compositions for producing olefins

1. A recombinant host cell engineered to produce an olefin, comprising a polynucleotide sequence encoding a polypeptide having at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 7, wherein the polypeptide has fatty acid decarboxylase activity.

2. The recombinant host cell of claim 1 , wherein the polynucleotide sequence is endogenous to the microorganism.

3. The recombinant host cell of claim 1 , wherein the polynucleotide sequence is heterologous to the microorganism.

4. The recombinant host cell of claim 1 , wherein the recombinant host cell expresses an increased level of the polypeptide having fatty acid decarboxylase activity relative to a corresponding wild-type host cell.

5. The recombinant host cell of claim 1 , wherein a gene encoding an acyl-CoA synthase is attenuated or deleted in the host cell and wherein the host cell expresses a reduced level of an acyl-CoA synthase relative to a corresponding wild type microorganism.

6. The recombinant host cell of claim 5 , wherein the acyl-CoA synthase gene is fadD.

7. The recombinant host cell of claim 4 , wherein the recombinant host cell is engineered to express, overexpress, or attenuate expression of a thioesterase.

8. A cell culture comprising the recombinant host cell of claim 4 , wherein an olefin is produced in the cell culture when the recombinant host cell is cultured in medium containing a substrate under conditions effective to express the polypeptide having fatty acid decarboxylase activity.

9. A cell culture comprising the recombinant host cell of claim 7 , wherein an olefin is produced in the cell culture when the recombinant host cell is cultured in medium containing a substrate under conditions effective to express the polypeptide having fatty acid decarboxylase activity.

10. The cell culture of claim 8 , wherein the olefin is a terminal olefin.

11. The cell culture of claim 8 , wherein the olefin is secreted by the host cell.

12. The cell culture of claim 10 , wherein the terminal olefin is selected from the group consisting of a C 5 -C 25 terminal olefin, a C 13 -C 21 terminal olefin, nonadecene, methylnonadecene, heptadecene, methylheptadecene, and pentadecene, an unsaturated terminal olefin, a monounsaturated terminal olefin, a straight chain terminal olefin, a branched chain terminal olefin, and a terminal olefin comprising a cyclic moiety.

13. The cell culture of claim 8 , wherein the substrate is a carbon source.

14. The cell culture of claim 13 , wherein the carbon source is a fatty acid.

15. The cell culture of claim 13 , wherein the carbon source is a carbohydrate.

16. The cell culture of claim 14 , wherein the fatty acid is selected from the group consisting of a C 6 -C 26 fatty acid, a C 14 -C 22 fatty acid, tetradecanoic acid, hexadecanoic acid, octadecanoic acid, behenic acid, methyl eicosanoic acid, eicosanoic acid, methyl octadecanoic acid, stearic acid, and palmitic acid, an unsaturated fatty acid, a monounsaturated fatty acid, a saturated fatty acid, a straight chain fatty acid, and a cyclic moiety.

17. A method of producing an olefin comprising culturing the host cell of claim 1 , under conditions sufficient to allow expression of the fatty acid decarboxylase polypeptide.

18. The method of claim 17 , further comprising culturing the host cell in the presence of at least one biological substrate for the polypeptide.

19. The method of claim 18 , further comprising isolating the olefin from the culture medium.

20. An olefin produced by the method of claim 19 , wherein the olefin has a δ 13 C selected from the group consisting of about −15.4 or greater, about −15.4 to about −10.9, and about −13.92 to about −13.84.

21. An olefin produced by the method of claim 19 , wherein the olefin has an f M 14 C selected from the group consisting of at least about 1.003, at least about 1.01, at least about 1.5, and about 1.111 to about 1.124.