Sequential analysis of biological samples
View Patent ↗Methods for detecting multiple targets in a biological sample are provided. The methods includes contacting the sample with a first probe; physically binding the first probe to a first target; observing a first signal from the first probe; applying a chemical agent to modify the first signal; contacting the sample with a second probe; physically binding the second probe to a second target; and observing a second signal from the second probe. The methods disclosed herein also provide for multiple iterations of binding, observing, signal modification for deriving information about multiple targets in a single sample. An associated kit and device are also provided.
1. A method of detecting multiple targets in a biological sample, comprising:
(a) contacting the biological sample with a first plurality of probes capable of binding a first group of different targets in the biological sample;
(b) binding the first plurality of probes to their respective targets;
(c) detecting signals from the first plurality of probes;
(d) applying an oxidizing agent after step (c) to modify the signals;
(e) contacting the biological sample after step (d) with a second plurality of probes capable of binding a second group of different targets in the biological sample;
(f) binding the second plurality of probes to their respective targets; and
(g) detecting signals from the second plurality of probes; and
wherein each probe comprises an antibody and a fluorophore.
2. The method of claim 1 , wherein the oxidizing agent is hydrogen peroxide.
3. The method of claim 1 , wherein the biological sample comprises a tissue section.