Methods for producing synthetic surfaces that mimic collagen coated surfaces for cell culture
View Patent ↗The present invention discloses methods for producing synthetic surfaces that mimic collagen coated surfaces for cell culture comprising: providing a monomer source comprising one or more organic compounds which are capable of polymerization, wherein at least one organic compound is prolinol; creating a plasma of said monomer source; and contacting at least a portion of a surface with the plasma to provide a plasma polymer coated surface. Advantageously, such methods provide an animal-free, synthetic, chemically defined surface that mimics a collagen coated surface for cell culture. Advantageously, such methods not only reduce the cost and/or issues associated with animal-derived collagen but are also amenable to large scale manufacturing.
1. A surface for cell culture wherein at least a portion of the surface comprises a coating of prolinol.
2. The surface of claim 1 , wherein prolinol is deposited onto the surface by plasma polymerization.
3. The surface of claim 1 , wherein prolinol is deposited onto the surface by chemical vapor deposition.
4. The surface of claim 1 , wherein prolinol is immobilized on the surface by covalent attachment to one or more carboxyl functional groups, one or more amine functional groups or a combination thereof.
5. The surface of claim 1 , wherein the surface mimics one or more functional characteristics of a collagen coated surface.
6. The surface of claim 1 , wherein human hepatocytes attach to the coating.
7. The surface of claim 1 , wherein the coating consists essentially of prolinol.