Bioreactor for syngas fermentation
View Patent ↗A bioreactor is provided that includes a main reactor having a configuration selected from the group consisting of stirred and unstirred tank reactor, trickle bed reactor (TBR), cocurrent contactor (CCC), moving bed bioreactor (MBBR), and a bubble column reactor. The bioreactor also includes a growth reactor continuous with the main reactor and having a configuration selected from the group consisting of stirred and unstirred tank reactor, trickle bed reactor (TBR), cocurrent contactor (CCC), moving bed bioreactor (MBBR), and a bubble column reactor. A method is also provided where acetogenic bacteria are contacted with syngas in a growth fermentor section of a reactor vessel that is continuous with a main fermentor section of a reactor vessel.
1. A process for fermentation of syngas, the process comprising:
filling at least about 75% of a total volume of a growth reactor section of a reactor vessel with medium, wherein a ratio of a main reactor volume to the growth reactor volume is about 10:1 to about 100:1;
contacting acetogenic bacteria with syngas in the growth section of the reactor vessel for a time effective for providing a cell density of at least about 3 grams per liter, wherein the growth section of the reactor vessel is configured as an unstirred tank reactor;
adding medium to the reactor vessel and maintaining a cell density of at least about 3 grams per liter until reaching a liquid level in a main section of the reactor vessel, wherein the growth section and main section of the reactor vessel are continuous;
introducing syngas into the main section of the reactor vessel through a gas sparger having holes of a diameter of 10 mm or less, the syngas being introduced at a flow rate effective for maintaining a pressure inside of the reactor vessel of at least about 1 psig, wherein the syngas has a CO/CO 2 molar ratio of at least about 0.75; and
providing an agitation energy input to the main section of the reactor vessel of about 0.01 to about 12 kWatts/m 3 medium, wherein the main section of the reactor vessel is configured as a stirred tank reactor,
wherein the process is effective for providing a volumetric CO mass transfer coefficient of about 100 to about 1500 per hour.
2. The process of claim 1 wherein the syngas pressure drop across the sparger is about 0.5 to about 2.5 psi.
3. The process of claim 1 wherein the process is effective for providing a space time yield of at least about 10 g ethanol/(L·day).
4. The process of claim 1 wherein the acetogenic bacteria is selected from the group consisting of Acetogenium kivui, Acetoanaerobium noterae, Acetobacterium woodii, Alkalibaculum bacchi CP11 (ATCC BAA-1772), Blautia producta, Butyribacterium methylotrophicum, Caldanaerobacter subterraneous, Caldanaerobacter subterraneous pacificus, Carboxydothermus hydrogenoformans, Clostridium aceticum, Clostridium acetobutylicum, Clostridium acetobutylicum P262 (DSM 19630 of DSMZ Germany), Clostridium autoethanogenum (DSM 19630 of DSMZ Germany), Clostridium autoethanogenum (DSM 10061 of DSMZ Germany), Clostridium autoethanogenum (DSM 23693 of DSMZ Germany), Clostridium autoethanogenum (DSM 24138 of DSMZ Germany), Clostridium carboxidivorans P7 (ATCC PTA-7827), Clostridium coskatii (ATCC PTA-10522), Clostridium drakei, Clostridium ljungdahlii PETC (ATCC 49587), Clostridium ljungdahlii ERI2 (ATCC 55380), Clostridium ljungdahlii C-01 (ATCC 55988), Clostridium ljungdahlii 0-52 (ATCC 55889), Clostridium magnum, Clostridium pasteurianum (DSM 525 of DSMZ Germany), Clostridium ragsdali P11 (ATCC BAA-622), Clostridium scatologenes, Clostridium thermoaceticum, Clostridium ultunense, Desulfotomaculum kuznetsovii, Eubacterium limosum, Geobacter sulfurreducens, Methanosarcina acetivorans, Methanosarcina barkeri, Morrella thermoacetica, Morrella thermoautotrophica, Oxobacter pfennigii, Peptostreptococcus productus, Ruminococcus productus, Thermoanaerobacter kivui , and mixtures thereof.