Platelet Additive Solution Having a Sialidase Inhibitor

1 . A platelet additive solution (PAS), comprising:

a) an amount of one or more sialidase inhibitors and optionally one or more glycan-modifying agents; and

b) one or more of PAS components that includes a salt, a citrate source, a carbon source, and any combination thereof.

2 . The PAS of claim 1 , wherein the PAS is maintained at a pH ranging between about 6.4 and about 7.6.

3 . The PAS of claim 1 , further including a phosphate source.

4 . The PAS of claim 3 , wherein the phosphate source is selected from the group consisting of sodium monophosphate, diphosphate, triphosphate, and a combination thereof.

5 . The PAS of claim 1 , wherein the citrate source is selected from the group consisting of monosodium citrate, disodium citrate, trisodium citrate, citric acid, and a combination thereof.

6 . The PAS of claim 1 , wherein the carbon source is selected from the group consisting of acetate, glucose and sucrose.

7 . The PAS of claim 6 , wherein the acetate is selected from the group consisting of sodium acetate, potassium acetate, magnesium acetate, and a combination thereof.

8 . The PAS of claim 1 , wherein the salt is selected from the group consisting of a sodium source, a chloride source, a potassium source, a magnesium source, a calcium source, and a combination thereof.

9 . The PAS of claim 8 , wherein the sodium source is selected from the group consisting of sodium chloride, sodium citrate, sodium acetate, sodium phosphate and a combination thereof.

10 . The PAS of claim 8 , wherein the chloride source is selected from the group consisting of sodium chloride, magnesium chloride, potassium chloride, and a combination thereof.

11 . The PAS of claim 8 , wherein the potassium source is selected from the group consisting of potassium chloride, potassium citrate, potassium acetate, potassium phosphate, potassium sulfate and a combination thereof.

12 . The PAS of claim 8 , wherein the magnesium source is selected from the group consisting of magnesium chloride, magnesium citrate, magnesium sulfate and a combination thereof.

13 . The PAS of claim 8 , wherein the calcium source is selected from the group consisting calcium chloride, calcium acetate, calcium citrate and a combination thereof.

14 . The PAS of claim 1 , wherein one or more of PAS components comprise:

a) a sodium source in the amount ranging between about 100 mM and about 300 mM;

b) a chloride source in the amount ranging between about 40 mM and about 110 mM;

c) a citrate source in the amount ranging between about 2 mM and about 20 mM;

d) an acetate source in the amount ranging between about 10 mM and about 50 mM;

e) a phosphate source in the amount ranging between about 5 mM and about 50 mM;

f) a potassium source in the amount ranging between about 0.5 mM and about 10 mM;

g) a magnesium source in the amount ranging between about 0.5 mM and about 2.5 mM; and

h) a calcium source in the amount ranging between about 0.5 mM and about 2.5 mM.

15 . A platelet composition comprising :

a) isolated platelets;

b) a PAS that comprises:

i) one or more sialidase inhibitors and optionally one or more glycan-modifying agents; and

ii) one or more of PAS components that includes a salt, a citrate source, a carbon source, and any combination thereof; and

c) plasma;

wherein the platelet composition is maintained at a pH ranging between about 6.4 and about 7.6.

16 . The platelet composition of claim 15 , wherein the plasma is present in an amount ranging between about 1% and about 50% by volume.

17 . The platelet composition of claim 15 , wherein the platelet additive solution is present in an amount ranging between about 50% and about 99% by volume.

18 . The platelet composition of claim 15 , wherein the one or more of PAS components comprises:

i) a sodium source in the amount ranging between about 100 mM and about 300 mM;

ii) a chloride source in the amount ranging between about 40 mM and about 110 mM;

iii) a citrate source in the amount ranging between about 2 mM and about 20 mM;

iv) an acetate source in the amount ranging between about 10 mM and about 50 mM;

v) a phosphate source in the amount ranging between about 5 mM and about 50 mM;

vi) a potassium source in the amount ranging between about 0.5 mM and about 10 mM;

vii) a magnesium source in the amount ranging between about 0.5 mM and about 2.5 mM;

viii) a calcium source in the amount ranging between about 0.5 mM and about 2.5 mM;

wherein the platelet composition is maintained at a pH ranging between about 6.4 and about 7.6.

19 . A bag or container that comprises:

a) a bag or container suitable for platelet storage; and

b) a PAS comprising:

i) an amount of one or more sialidase inhibitors and optionally one or more glycan-modifying agents; and

ii) one or more of PAS components that includes a salt, a citrate source, a carbon source, and any combination thereof.

20 . The bag or container of claim 19 , further comprising isolated platelets.

21 . The bag or container of claim 19 , wherein the PAS is maintained at a pH ranging between about 6.4 and about 7.6.

22 . The bag or container of claim 19 , wherein one or more of PAS components

i) a sodium source in the amount ranging between about 100 mM and about 300 mM;

ii) a chloride source in the amount ranging between about 40 mM and about 110 mM;

iii) a citrate source in the amount ranging between about 2 mM and about 20 mM;

iv) an acetate source in the amount ranging between about 10 mM and about 50 mM;

v) a phosphate source in the amount ranging between about 5 mM and about 50 mM;

vi) a potassium source in the amount ranging between about 0.5 mM and about 10 mM;

vii) a magnesium source in the amount ranging between about 0.5 mM and about 2.5 mM;

viii) a calcium source in the amount ranging between about 0.5 mM and about 2.5 mM;

wherein the platelet composition is maintained at a pH ranging between about 6.4 and about 7.6.

23 . The bag or container of claim 19 , further comprising isolated platelets.

24 . A method of storing platelets, wherein isolated platelets are obtained from one or more donors, the method comprises:

a) contacting the isolated platelets with a PAS that comprises:

i) an amount of one or more sialidase inhibitors and optionally one or more glycan-modifying agents; and

ii) one or more of PAS components that includes a salt, a citrate source, a carbon source, and any combination thereof.

25 . The method of claim 24 , wherein the sialidase inhibitor is selected from the group consisting of: fetuin, 2,3-dehydro-2-deoxy-N-acetylneuraminic acid (DANA) or a pharmaceutically acceptable salt thereof; ethyl (3R,4R,5S)-5-amino-4-acetamido-3-(pentan-3-yloxy)-cyclohex-1-ene-1-carboxylate); (2R,3R,4S)-4-guanidino-3-(prop-1-en-2-ylamino)-2-((1R,2R)-1,2,3-trihydroxypropyl)-3,4-dihydro-2H-pyran-6-carboxylic acid; (4S,5R,6R)-5-acetamido-4-carbamimidamido-6-[(1R,2R)-3-hydroxy-2-methoxypropyl]-5,6-dihydro-4H-pyran-2-carboxylic acid; and (1S,2S,3S,4R)-3-[(1S)-1-acetamido-2-ethyl-butyl]-4-(diaminomethylideneamino)-2-hydroxy-cyclopentane-1-carboxylic acid, or a pharmaceutically acceptable salt thereof.

26 . The method of claim 25 , wherein the sialidase inhibitor is the sodium salt of 2,3-dehydro-2-deoxy-N-acetylneuraminic acid.

27 . The method of claim 24 , wherein the isolated platelets are stored for a period of about 1 to about 21 days.

28 . The method of claim 24 , wherein the isolated platelets are stored a temperature of between about 2° C. and about 25° C.

29 . The method of claim 24 , further comprising cooling the platelet composition to a temperature below room temperature; storing the platelet composition for a period of time; and then rewarming the platelet composition back to room temperature.

30 . The method of claim 24 , further including treating the population of platelets with the sialidase inhibitor within a time period, wherein the time period is in a range between about 1 minute to about 8 hours.