Placental stem cell populations
View Patent ↗The present invention provides placental stem cells and placental stem cell populations, and methods of culturing, proliferating and expanding the same. The invention also provides methods of differentiating the placental stem cells. The invention further provides methods of using the placental stem cells in assays and for transplanting.
1. A population of isolated adherent placental stem cells, wherein said placental stem cells:
express genes at a detectably higher level than an equivalent number of bone marrow-derived mesenchymal stem cells (BM-MSCs); wherein said genes comprise CPA4, TCF21, VTN, B4GALT6, FLJ10781, and NUAK1; wherein at least 70% of said placental stem cells are non-maternal in origin:
and wherein said placental stem cells have been passaged between 3 and 10 times.
2. The population of claim 1 , wherein said placental stem cells express said genes at a detectably higher level than an equivalent number of BM-MSCs in medium comprising DMEM-LG and MCDB-201; 2% fetal calf serum, 1× insulin-transferrin-selenium, 1× lenolenic-acid-bovine-serum-albumin, dexamethasone, 10 −4 M ascorbic acid 2-phosphate, 10 ng/ml epidermal growth factor, and 10 ng/ml platelet derived-growth factor.
3. The population of claim 1 , wherein said population comprises from 10 6 to 10 7 placental stem cells.
4. The population of claim 1 , wherein said population comprises from 10 7 to 10 8 placental stem cells.
5. A pharmaceutical composition comprising the population of claim 1 , in a pharmaceutically acceptable carrier.
6. The pharmaceutical composition of claim 5 , wherein said population comprises from 10 6 to 10 7 placental stem cells.
7. The pharmaceutical composition of claim 5 , wherein said population comprises from 10 7 to 10 8 placental stem cells.
8. The pharmaceutical composition of claim 5 , wherein said pharmaceutical composition is an injectable solution.
9. A pharmaceutical composition comprising the population of claim 2 , in a pharmaceutically acceptable carrier.
10. The population of claim 1 , wherein at least 90% of said placental stem cells are non-maternal in origin.
11. The population of claim 1 , wherein said genes further comprise CD200.
12. The population of claim 1 , wherein said genes further comprise ARTS-1, IER3, IL6, KRT18, LRAP, MEST, NFE2L3, or TGFB2.
13. The population of claim 1 , wherein said expression of said genes is detectably higher level than an equivalent number of bone marrow-derived mesenchymal stem cells over 3 population doublings.
14. The population of claim 1 , wherein said expression of said genes is detectably higher level than an equivalent number of bone marrow-derived mesenchymal stem cells over 11-14 population doublings.
15. The population of claim 1 , wherein said expression of said genes is detectably higher level than an equivalent number of bone marrow-derived mesenchymal stem cells over 24-38 population doublings.
16. The population of claim 1 , wherein said population has undergone at least 5 population doublings.
17. The population of claim 1 , wherein said population has undergone between 15 and 30 population doublings.
18. The population of claim 1 , wherein said population has undergone about 20 population doublings.
19. The population of claim 1 , additionally comprising stem cells that are not obtained from placental tissue.
20. The population of claim 1 , wherein said placental stem cells have been cryopreserved.
21. The population of claim 1 , wherein said placental stem cells have the ability to replicate 10-40 times in culture.
22. The population of claim 1 , wherein said placental stem cells are isolated from a human postpartum placenta by digestion using trypsin, or are cultured from cells isolated from a human postpartum placenta by digestion using trypsin.
23. The population of claim 1 , wherein said placental stem cells have been separated from at least 90% of cells from the placenta from which the placental stem cells are isolated.
24. The population of claim 1 , wherein said placental stem cells have been separated from at least 95% of cells from the placenta from which the placental stem cells are isolated.
25. The population of claim 24 , wherein said placental stem cells have been separated from at least 99% of cells from the placenta from which the placental stem cells are isolated.
26. The population of claim 1 , wherein said placental stem cells have been passaged 6 times.
27. The population of claim 1 , wherein said population is present in a composition comprising dimethylsulfoxide (DMSO), human serum albumin (HSA), or dextran.
28. The population of claim 1 , wherein at least 90% of cells in the population are said placental stem cells.
29. The population of claim 1 , wherein at least 95% of cells in the population are said placental stem cells.
30. The population of claim 1 , wherein at least 99% of cells in the population are said placental stem cells.
31. The population of claim 1 , wherein said placental stem cells express said genes at at least a three-fold higher level than an equivalent number of BM-MSCs.