Restriction endonucleases, DNA encoding these endonucleases and methods for indentifying new endonucleases with the same or varied specificity
View Patent ↗Specified restriction endonucleases have been characterized for the first time by their amino acid and DNA sequences. These sequences and those with at least 90% identity thereto have been used as probes in sequence similarity analyses to identify sequence matches in a sequence database that corresponds to novel restriction endonucleases or isoschizomers. The sequence similarity analyses includes selecting a positive sequence match from any sequence producing an expectation value of less than or equal to e−02.
1. An isolated protein having at least 90% amino acid sequence identity with SEQ ID NO: 14.
2. An isolated protein having an amino acid sequence identified by an Expectation value of less than or equal to e−05 in a BLAST search for detecting sequence similarity using the amino acid sequence of SEQ ID NO:14.
3. The isolated protein according to claim 2 , having restriction endonuclease activity.
4. The isolated protein of claim 1 , wherein the protein is present in a buffer.
5. The isolated protein of claim 2 , wherein the protein is present in a buffer.