HERBICIDE RESISTANT ORGANISMS
Disclosed herein are transformed non-vascular photosynthetic organisms that are herbicide resistant, nucleotides and vectors useful in conducting such transformations, and transformed strains produced by such transformations.
1 - 168 . (canceled)
169 . A non-vascular photosynthetic organism comprising at least one mutation in a nucleotide sequence of any one of SEQ ID. NO. 74, SEQ ID. NO. 86, SEQ ID NO. 102, SEQ ID NO. 62, SEQ ID NO. 63, SEQ ID NO. 64, SEQ ID NO. 65, SEQ ID NO. 66, SEQ ID NO. 67, SEQ ID NO. 68, SEQ ID NO. 69, SEQ ID NO. 70, SEQ ID NO. 71, SEQ ID NO. 72, SEQ ID NO. 85, SEQ ID NO. 119, SEQ ID NO. 120, SEQ ID NO. 121, SEQ ID NO. 122, SEQ ID NO. 123, SEQ ID NO. 124, SEQ ID NO. 125, SEQ ID NO. 126 and SEQ ID NO. 127 or a sequence having at least 95% identity thereto; wherein the at least one mutation comprises one or more nucleotide additions, deletions or substitutions; and wherein the non-vascular photosynthetic organism has an increased growth rate in the presence of a glyphosate herbicide as compared to a non-vascular photosynthetic organism without the at least one mutation.
170 . The non-vascular photosynthetic organism of claim 169 , wherein the growth rate of the non-vascular photosynthetic organism is at least 10%, at least 20%, at least 40%, at least 60%, at least 80%, at least 100%, at least 150%, at least 200%, at least 250%, at least 300%, at least 350%, at least 400%, at least 450%, or at least 500% greater than the non-vascular photosynthetic organism without the at least one mutation.
171 . The non-vascular photosynthetic organism of claim 169 , wherein the at least one mutation results in one or more amino acid additions, deletions or substitutions M a protein encoded by the nucleotide sequence.
172 . The non-vascular photosynthetic organism of claim 169 , wherein transcription of the nucleotide sequence is decreased as compared to transcription in the non-vascular photosynthetic organism without the at least one mutation.
173 . The non-vascular photosynthetic organism of claim 172 , wherein the transcription is decreased by at least 10%, at least 20%, at least 30%, at least 40%, at least 50%, at least 60%, at least 70%, at least 80%, at least 90% or 100%.
174 . The non-vascular photosynthetic organism of claim 169 , wherein the organism is an alga.
175 . The non-vascular photosynthetic organism of claim 174 , wherein the alga is a Chlamydomonas sp., Volvacales sp., Dunaliella sp., Scenedesmus sp., Chlorella sp., Hematococcus sp., Volvox sp., or Nannochloropsis sp.
176 . The non-vascular photosynthetic organism of claim 169 , wherein the organism is a cyanobacterium.
177 . The non-vascular photosynthetic organism of claim 169 , wherein the organism is grown in an aqueous environment.
178 . The non-vascular photosynthetic organism of claim 177 , wherein the concentration of glyphosate or glyphosate herbicide in the aqueous environment is between 0.5 mM and 6.5 mM, or between 1 mM and 5 mM, or between 2.5 mM and 5 mM, or is about 0.5 mM, about 1 mM, about 1.5 mM, about 2 mM, about 2.5 mM, about 3 mM, about 3.5 mM, about 4 mM, about 4.5 mM, about 5 mM, about 5.5 mM, about 6 mM, or about 6.5 mM.
179 . A genetically modified non-vascular photosynthetic organism comprising at least one RNAi agent, the at least one RNAi agent comprising an antisense nucleotide sequence that is complementary to mRNA transcribed from any one of SEQ ID NO. 74, SEQ ID NO. 86, SEQ ID NO. 102, SEQ ID NO. 62, SEQ ID NO. 63, SEQ ID NO. 64, SEQ ID NO. 65, SEQ ID NO. 66, SEQ ID NO. 67, SEQ ID NO. 68, SEQ ID NO. 69, SEQ ID NO. 70, SEQ ID NO. 71, SEQ ID NO. 72, SEQ ID NO. 85, SEQ ID NO. 119, SEQ ID NO. 120, SEQ ID NO. 121, SEQ ID NO. 122, SEQ ID NO. 123, SEQ. ID NO. 124, SEQ. ID NO. 125, SEQ ID NO. 126, and SEQ. ID NO. 127 or a sequence having at least 95% identity thereto; and wherein the non-vascular photosynthetic organism has an increased growth rate in the presence of a glyphosate herbicide as compared to a non-vascular photosynthetic organism not modified with the at least one RNAi agent.
180 . The non-vascular photosynthetic organism of claim 179 , wherein the growth rate of the non-vascular photosynthetic organism is at least 10%, at least 20%, at least 40%, at least 60%, at least 80%, at least 100%, at least 150%, at least 200%, at least 250, at least 300%, at least 350%, at least 400%, at least 450, or at least 500% greater than the non-vascular photosynthetic organism not modified with the at least one RNAi agent.
181 . The non-vascular photosynthetic organism of claim 179 , wherein the at least one RNAi agent is a microRNA (miRNA) or is a small interfering RNA (siRNA).
182 . The non-vascular photosynthetic organism of claim 179 , wherein full length transcript levels of any one of SEQ ID NO. 74, SEQ ID NO. 86, SEQ NO. 102, SEQ ID NO. 62, SEQ ID NO. 63, SEQ ID NO. 64, SEQ ID NO. 65, SEQ ID NO. 66, SEQ ID NO. 67, SEQ ID NO. 68, SEQ. ID NO. 69, SEQ. ID NO. 70, SEQ. ID NO. 71, SEQ. ID NO. 72, SEQ. ID NO. 85, SEQ. ID NO. 119, SEQ ID NO. 120, SEQ ID NO. 121, SEQ ID NO. 122, SEQ ID NO. 123, SEQ ID NO. 124, SEQ ID NO. 125, SEQ ID NO. 126, and SEQ ID NO. 127 or a sequence having at least 95% identity thereto, are decreased as compared to the non-vascular photosynthetic organism not modified with the at least one RNAi agent.
183 . The non-vascular photosynthetic organism of claim 182 , wherein the transcripts are decreased by at least 10%, at least 20%, at least 30%, at least 40%, at least 50%, at least 60%, at least 70%, at least 80%, at least 90% or by 100%.
184 . The non-vascular photosynthetic organism of claim 179 , wherein the organism is an alga.
185 . The non-vascular photosynthetic organism of claim 184 , wherein the alga is a Chlamydomonas sp., Volvacales sp., Dunaliella sp., Scenedesmus sp., Chlorella sp., Hematococcus sp., Volvox sp., or Nannochloropsis sp.
186 . The non-vascular photosynthetic organism of claim 179 , wherein the organism is a cyanobacterium.
187 . The non-vascular photosynthetic organism of claim 179 , wherein the organism is grown in an aqueous environment.
188 . The non-vascular photosynthetic organism of claim 187 , wherein the concentration of glyphosate or glyphosate herbicide in the aqueous environment is between 0.5 mM and 6.5 mM, or between 1 mM and 5 mM, or between 2.5 mM and 5 mM, or is about 0.5 mM, about 1 mM, about 1.5 mM, about 2 mM, about 2.5 mM, about 3 mM., about 3.5 mM, about 4 mM, about 4.5 mM, about 5 mM, about 5.5 mM, about 6 mM, or about 6.5 mM.