Acetyl CoA carboxylases
View Patent ↗Provided herein are novel ACCases and nucleotides encoding the same, that when introduced into a cell or organism results in an increase and/or accumulation of fatty acids, glycerol lipids, and/or oils in the cell or organism, and/or a change in the types of fatty acids, glycerol lipids, and/or oils that are normally present in the cell or organism. Also provided herein are organisms transformed with the novel ACCases.
1. A non-vascular photosynthetic organism transformed with a polynucleotide, comprising a nucleic acid sequence that encodes a protein comprising the amino acid sequence of SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 23, or SEQ ID NO: 24.
2. The non-vascular photosynthetic organism of claim 1 , wherein the nucleic acid sequence comprises: the nucleotide sequence of SEQ ID NO: 29, SEQ ID NO: 30, SEQ ID NO: 27, SEQ ID NO: 28, SEQ ID NO: 31, SEQ ID NO: 32, SEQ ID NO: 33, SEQ ID NO: 34, SEQ ID NO: 35, or SEQ ID NO: 36; or a sequence that has at least 80%, at least 90%, at least 95%, at least 98%, or at least at least 99% sequence identity to the nucleotide sequence of SEQ ID NO: 29, SEQ ID NO: 30, SEQ ID NO: 27, SEQ ID NO: 28, SEQ ID NO: 31, SEQ ID NO: 32, SEQ ID NO: 33, SEQ ID NO: 34, SEQ ID NO: 35, or SEQ ID NO: 36.
3. The non-vascular photosynthetic organism of claim 1 , wherein the non-vascular photosynthetic organism is an alga.
4. The non-vascular photosynthetic organism of claim 3 , wherein the alga is a Chlorophycean species.
5. The non-vascular photosynthetic organism of claim 1 , wherein the non-vascular photosynthetic organism is a cyanobacterium.
6. A non-vascular photosynthetic organism transformed with a nucleotide sequence comprising SEQ ID NO: 168 or SEQ ID NO: 169; or a nucleotide sequence that has at least 80%, at least 90%, at least 95%, at least 98%, or at least at least 99% sequence identity to the nucleic acid sequence of SEQ ID NO: 168 or SEQ ID NO: 169.
7. The non-vascular photosynthetic organism of claim 6 , wherein the non-vascular photosynthetic organism is an alga.
8. The non-vascular photosynthetic organism of claim 7 , wherein the alga is a Chlorophycean species.
9. The non-vascular photosynthetic organism of claim 6 , wherein the non-vascular photosynthetic organism is a cyanobacterium.
10. A non-vascular photosynthetic organism transformed with a polynucleotide, comprising: a nucleic acid sequence that encodes a protein comprising the amino acid sequence of SEQ ID NO: 157; or a sequence that has at least 75%, at least 80%, at least 90%, at least 95%, at least 98%, or at least at least 99% sequence identity to the amino acid sequence of ID NO: 157.
11. The non-vascular photosynthetic organism of claim 10 , wherein the nucleic acid sequence comprises: the nucleotide sequence of SEQ ID NO: 114 or SEQ ID NO: 115; or a sequence that has at least 75%, at least 80%, at least 90%, at least 95%, at least 98%, or at least at least 99% sequence identity to the nucleotide sequence of SEQ ID NO: 114 or SEQ ID NO: 115.
12. The non-vascular photosynthetic organism of claim 10 , wherein the non-vascular photosynthetic organism is an alga.
13. The non-vascular photosynthetic organism of claim 12 , wherein the alga is a Chlorophycean species.
14. The non-vascular photosynthetic organism of claim 10 , wherein the non-vascular photosynthetic organism is a cyanobacterium.
15. A method for increasing production of malonyl CoA in a non-vascular photosynthetic organism, comprising transforming the non-vascular photosynthetic organism with a polynucleotide encoding a protein comprising the amino acid sequence of: SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 23, SEQ ID NO: 24, SEQ ID NO: 163, SEQ ID NO: 164, SEQ ID NO: 165, SEQ ID NO: 166, SEQ ID NO: 167, or SEQ ID NO: 157, wherein expression of the protein results in increased production of malonyl CoA in the non-vascular photosynthetic organism.
16. The method of claim 15 , wherein the non-vascular photosynthetic organism is an alga.
17. The method of claim 16 , wherein the alga is a Chlorophycean species.
18. method of claim 15 , wherein the non-vascular photosynthetic organism is a cyanobacterium.