METHODS AND APPARATUS FOR INTRODUCING CELLS AT A TISSUE SITE
The present invention relates to methods and devices for maintaining cellular viability and function for therapeutic purposes. The invention provides methods and devices for maintaining the proliferative and developmental potential of cellular preparations by protecting the from physical and physiological damage during storage, preparation, and delivery to a site (e.g., a tissue site). The invention also provides methods and devices for evaluating tissues and organs, and selecting appropriate sites for cellular delivery.
1 . A system for introducing cells into a tissue, comprising:
a working end of a cell introducing device having at least one opening for releasing cells at a tissue site.
2 . A method for introducing cells into a tissue, comprising:
releasing cells from an opening of a working end of a cell introducing device at a tissue site.
3 - 40 . (canceled)
41 . The method of claim 2 , wherein a cell preparation is warmed to a threshold temperature within a predetermined time period prior to injection.
42 . The method of claim 41 , wherein the predetermined time period is 1 second −60 minutes.
43 . The method of claim 41 , wherein the threshold temperature is between liquid nitrogen temperature and body temperature.
44 . The method of claim 43 , wherein the threshold temperature is 5-10 degrees centigrade below body temperature.
45 . The method of claim 2 , wherein the cells are released below or above a threshold pressure at the tissue site.
46 . The method of claim 45 , wherein the threshold pressure is between 100 and 200 mm mercury.
47 . The method of claim 45 , wherein the threshold pressure is greater or less than the blood pressure of the recipient.
48 - 50 . (canceled)
51 . The system of claim 1 , further comprising a microfluidic circuit in fluid connection with the working end of the cell introducing device.
52 . The system of claim 51 , wherein the microfluidic circuit comprises a gas-permeable membrane, ceramic porous material, metallic fritted material.
53 . The system of claim 51 , wherein the microfluidic circuit comprises one or more sensors selected from the group consisting of sensors for pH, oxygen, carbon dioxide, temperature, pressure, or specific degradation products of cellular metabolism.
54 . The system of claim 51 , further comprising a working end that is 0.001-250 mm long.
55 - 57 . (canceled)
58 . A method for delivering infrared light into a tissue, the method comprising piercing the tissue surface using a device that has an insertable member that conducts infrared light, and transmitting infrared light through the insertable member into the tissue.
59 - 61 . (canceled)