Lipoaspirate stem cell separation system and methods thereof

1. A system for isolating and separating lipoaspirate particles, comprising:

a generally cylindrical container having a lid and a bottom wherein the generally cylindrical container includes an input port positioned to permit a lipoaspirate fluid to enter the generally cylindrical container above the bottom and a shelf fixed to a wall of the generally cylindrical container between the input port and the bottom, wherein the input port is directed into a side of the generally cylindrical container with a central axis of the input port oriented at an angle that is almost tangential to the side of the generally cylindrical container where the input port enters the generally cylindrical container, wherein the shelf comprises an open central portion, and wherein the shelf is slanted such that an inner diameter of the shelf is disposed at a higher elevation than an outer diameter of the shelf;

a source of a vacuum coupled to the generally cylindrical container to provide a partial vacuum during use of the system; and

an expansion chamber coupled to the input port.

2. The system of claim 1 , including at least one exit port in the generally cylindrical container for removing separated lipoaspirate fluid.

3. The system of claim 1 , including at least one mesh shelf in the generally cylindrical container below the shelf.

4. The system of claim 1 , wherein the input port is positioned in an upper half of the generally cylindrical container.

5. A method of separating lipoaspirate particles, comprising:

providing a cylindrical container having a lid and a bottom wherein the cylindrical container includes at least one input port positioned to permit a lipoaspirate fluid to enter the cylindrical container above the bottom, wherein the input port is directed into a side of the cylindrical container with a central axis of the input port oriented at an angle that is almost tangential to the side of the cylindrical container where the input port enters the cylindrical container, and wherein the cylindrical container includes a shelf fixed to a wall of the cylindrical container, the shelf having an open central portion, and wherein the shelf is slanted such that an diameter part of the shelf is disposed at a higher elevation than an outer diameter of the shelf;

providing a source of a vacuum coupled to the cylindrical container to provide a partial vacuum during use of the cylindrical container;

creating a partial vacuum within the cylindrical container to pull fresh lipoaspirate particles through the input port so that the fresh lipoaspirate particles flows into the cylindrical container; and

wherein the cylindrical container further comprises an expansion chamber coupled to the input port.

6. The method of claim 5 , including wherein the cylindrical container includes at least one exit port in the cylindrical container for removing separated lipoaspirate fluid.

7. The method of claim 5 , wherein the cylindrical container includes at least one mesh shelf in the cylindrical container below the shelf.

8. The method of claim 5 , wherein the input port is positioned in an upper half of the cylindrical container.

9. The method of claim 5 , wherein the input port comprises a tube affixed to an inner wall of the cylindrical container for lateral delivery/injection of entering lipoaspirate fluid onto the inner wall of the cylindrical container.

10. A system for isolating and separating lipoaspirate particles comprising:

a generally cylindrical container having a lid and a bottom wherein the generally cylindrical container includes at least one input port positioned to permit a lipoaspirate fluid to enter the generally cylindrical container above the bottom;

a cone disposed within the generally cylindrical container, the cone comprising a tip pointing up toward the input port;

a ring-shaped shelf fixed to a wall of the generally cylindrical container and disposed between the cone and the bottom of the generally cylindrical container, the ring-shaped shelf comprising an inner diameter defining an open central portion and an outer diameter that is greater than the inner diameter;

a source of a vacuum coupled to the generally cylindrical container to provide a partial vacuum during use of the system; and

an expansion chamber coupled to the input port.

11. The system of claim 10 , wherein a relative height of the inner diameter of the ring-shaped shelf is higher than the outer diameter of the ring-shaped shelf.

12. The system of claim 10 , including at least one mesh shelf in the generally cylindrical container below the ring-shaped shelf.

13. The system of claim 10 , wherein a bottom diameter of the cone is larger than the inner diameter of the ring-shaped shelf.

14. The system of claim 10 , wherein the cone comprises perforations to permit equalization of pressure between an area of the generally cylindrical container beneath the cone with an area of the generally cylindrical container above the cone.