IP Library › Granted Patent US 9,464,333
Granted Patent B2
US 9,464,333 · App. 13/508,234 · Granted Oct 11, 2016

Intein-modified enzymes, their production and industrial applications

Inventors: James Apgar (Somerville, MA); Philip Lessard (Framingham, MA); R. Michael Raab (Arlington, MA); Binzhang Shen (Jamaica, MA); Gabor Lazar (Belmont, MA); Humberto de la Vega (Peabody, MA)
Assignee: Agrivida, Inc.
C12Y302/01004C12N9/248C12N9/2437C12N15/8216C12N15/8246C12Y302/01008
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Quick Facts
Patent No.
US 9,464,333
App. No.
13/508,234
Granted
Oct 11, 2016
Kind
B2
Abstract

A method of predicting an intein insertion site in a protein that will lead to a switching phenotype is provided. The method includes identifying a plurality of C/T/S sites within the protein; selecting from the plurality of C/T/S/ sites those that are ranked 0.75 or higher by a support vector machine, within ten angstroms of the active site of the protein, and at or near a loop-β-sheet junction or a loop-α-helix junction. A method of controlling protein activity and hosts including proteins with controlled activity are also provided. Also, intein modified proteins and plants containing intein modified proteins are provided.

Claims (6)

1. An intein modified protein comprising an amino acid sequence selected from the group consisting of SEQ ID NOS: 2374, 2376-2378, 2383, 2385-2386, 2410, 2412-2413, 2420, 2422-2425, 2428, 2430-2431, 2436, 2442-2444, 2451, 2454-2458, 2460-2461, 2465-2468, 2471-2474, 2477-2480, 2482-2483, 2493-2494, 2500-2501, 2504, 2507-2513, 2517-2519, 2530-2531, 2533-2537, 2539-2543, 2545, 2548-2549, 2555-2557, 2559, 2565, 2569-2571, 2573-2575, 2579-2582, 2584-2597, 2600, 2602-2605, 2607-2621, 2624-2626, 2629-2634, 2636-2639, 2643, 2645-2648, 2650, 2652, 2656-2658, 2661, 2664, 2666-2672, 2674, 2677-2683, 2685, and 3315-3322.

2. A nucleic acid comprising a nucleotide sequence encoding an intein modified protein comprising an amino acid sequence selected from the group consisting of SEQ ID NOS: 2374, 2376-2378, 2383, 2385-2386, 2410, 2412-2413, 2420, 2422-2425, 2428, 2430-2431, 2436, 2442-2444, 2451, 2454-2458, 2460-2461, 2465-2468, 2471-2474, 2477-2480, 2482-2483, 2493-2494, 2500-2501, 2504, 2507-2513, 2517-2519, 2530-2531, 2533-2537, 2539-2543, 2545, 2548-2549, 2555-2557, 2559, 2565, 2569-2571, 2573-2575, 2579-2582, 2584-2597, 2600, 2602-2605, 2607-2621, 2624-2626, 2629-2634, 2636-2639, 2643, 2645-2648, 2650, 2652, 2656-2658, 2661, 2664, 2666-2672, 2674, 2677-2683, 2685, and 3315-3322.

3. A transgenic plant comprising the nucleic acid of claim 2 .

4. The transgenic plant of claim 3 further comprising an expression construct, wherein the nucleic acid is contained within the expression construct.

5. The transgenic plant of claim 4 , wherein the expression construct comprises the sequence of SEQ ID NO: 1.

6. The intein modified protein of claim 1 comprising the amino acid sequence of SEQ ID NO: 3315.

Assignments (4)
MERGER Recorded Nov 9, 2023
From: AGRIVIDA MERGER SUB, INC.; AGRIVIDA, INC.
To: AGRIVIDA INC.
Reel/Frame 065513/0941 →
CONFIRMATORY LICENSE Recorded Dec 29, 2015
From: AGRIVIDA, INC.
To: U.S. DEPARTMENT OF ENERGY
Reel/Frame 037375/0194 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Dec 19, 2014
From: SHEN, BINZHANG; LAZAR, GABOR; DE LA VEGA, HUMBERTO; LESSARD, PHILIP; RAAB, R. MICHAEL; APGAR, JAMES
To: AGRIVIDA, INC.
Reel/Frame 034560/0163 →
SECURITY AGREEMENT Recorded May 7, 2013
From: AGRIVIDA INC.
To: TRIPLEPOINT CAPITAL LLC
Reel/Frame 030370/0134 →
Continuity (2)
Continuation In Part 12590444 · Nov 6, 2009
Related Publication 20130036517A1 · Feb 7, 2013