Granted Patent
B2
US 8,759,293 · App. 13/509,309 · Granted Jun 24, 2014
von Willebrand factor (vWF)-containing preparations, and methods, kits, and uses related thereto
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Abstract
The present invention relates to methods, compositions and kits for preparing FVIII and employing same. Also provided are vWF polypeptides and nucleic acid molecules encoding same.
Claims (10)
1. An isolated von Willebrand factor (vWF) polypeptide comprising a Factor VIII (FVIII) binding domain, with the proviso that the polypeptide lacks one of vWF domain A1, A2, A3, D4, B1, B2, B3, C1, C2, CK, or a combination thereof, wherein the polypeptide further comprises an immunoglobulin Fc amino acid sequence comprising at least a hinge region, wherein the polypeptide exhibits binding to the FVIII, wherein the Factor VIII (FVIII) binding domain comprises the amino acid sequence as set forth in SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:33, SEQ ID NO:34, or SEQ ID NO:35.
2. An isolated von Willebrand factor (vWF) polypeptide comprising a Factor VIII (FVIII) binding domain, with the proviso that the polypeptide lacks one of vWF domain A1, A2, A3, D4, B1, B2, B3, C1, C2, CK, or a combination thereof, wherein the polypeptide further comprises an immunoglobulin Fc amino acid sequence comprising at least a hinge region, wherein the polypeptide exhibits binding to the FVIII, wherein the polypeptide comprises the sequence set forth in SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:36, SEQ ID NO:38, or SEQ ID NO:39.
3. The polypeptide of claim 1 , wherein the polypeptide is capable of forming a dimer.
4. The polypeptide of claim 1 , wherein the immunoglobulin Fc amino acid sequence has the sequence set forth in SEQ ID NO:16.
5. A composition comprising the polypeptide of claim 1 .
6. A protein complex comprising the polypeptide of claim 1 bound to the FVIII.
7. A composition comprising the protein complex of claim 6 .
8. A cell expressing the polypeptide of claim 1 .
9. A cell expressing the protein complex of claim 6 .
10. A composition comprising the protein complex of claim 6 , and a pharmaceutically acceptable carrier.
Assignments (29)
RELEASE OF SECURITY INTEREST IN PATENTS, RECORDED ON DECEMBER 10, 2021 AT REEL 058480 FRAME 0221
Recorded May 21, 2026
From: BANK OF AMERICA, N.A., AS COLLATERAL AGENT
To: GRIFOLS SHARED SERVICES NORTH AMERICA, INC.; GRIFOLS THERAPEUTICS LLC (FORMERLY KNOWN AS GRIFOLS THERAPEUTICS, INC.); GRIFOLS BIOLOGICALS LLC (FORMERLY KNOWN AS GRIFOLS BIOLOGICALS, INC.); BIOMAT USA, INC.; GRIFOLS USA, LLC; GRIFOLS WORLDWIDE OPERATIONS USA, INC.; TALECRIS PLASMA RESOURCES, INC.
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From: EUROPEAN INVESTMENT BANK
To: GRIFOLS SHARED SERVICES NORTH AMERICA, INC.; GRIFOLS THERAPEUTICS LLC (F/K/A GRIFOLS THERAPEUTICS, INC.); GRIFOLS BIOLOGICALS LLC (F/K/A GRIFOLS BIOLOGICALS, INC.); BIOMAT USA, INC.; GRIFOLS WORLDWIDE OPERATIONS USA, INC.; GRIFOLS USA, LLC; TALECRIS PLASMA RESOURCES, INC.
Reel/Frame 075416/0698 →
RELEASE OF SECURITY INTEREST
Recorded Apr 15, 2026
From: EUROPEAN INVESTMENT BANK
To: GRIFOLS SHARED SERVICES NORTH AMERICA, INC.; GRIFOLS THERAPEUTICS LLC (F/K/A GRIFOLS THERAPEUTICS, INC.); GRIFOLS BIOLOGICALS LLC (F/K/A GRIFOLS BIOLOGICALS, INC.); BIOMAT USA, INC.; GRIFOLS WORLDWIDE OPERATIONS USA, INC.; GRIFOLS USA, LLC; TALECRIS PLASMA RESOURCES, INC.
Reel/Frame 075416/0718 →
RELEASE OF SECURITY INTEREST
Recorded Apr 15, 2026
From: EUROPEAN INVESTMENT BANK
To: GRIFOLS SHARED SERVICES NORTH AMERICA, INC.; GRIFOLS THERAPEUTICS LLC (F/K/A GRIFOLS THERAPEUTICS, INC.); GRIFOLS BIOLOGICALS LLC (F/K/A GRIFOLS BIOLOGICALS, INC.); BIOMAT USA, INC.; GRIFOLS WORLDWIDE OPERATIONS USA, INC.; GRIFOLS USA, LLC; TALECRIS PLASMA RESOURCES, INC.
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From: THE BANK OF NEW YORK MELLON, LONDON BRANCH
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From: GRIFOLS SHARED SERVICES NORTH AMERICA, INC.; GRIFOLS THERAPEUTICS LLC; GRIFOLS BIOLOGICALS LLC; GRIFOLS WORLDWIDE OPERATIONS USA, INC.; GRIFOLS USA, LLC
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From: GRIFOLS SHARED SERVICES NORTH AMERICA, INC.; GRIFOLS THERAPEUTICS INC.; GRIFOLS BIOLOGICALS INC.; BIOMAT USA, INC.; GRIFOLS DIAGNOSTIC SOLUTIONS.; GRIFOLS WORLDWIDE OPERATIONS USA, INC.; GRIFOLS USA, LLC
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