HUMAN ARTIFICIAL CHROMOSOME VECTOR
A human artificial chromosome vector comprising a human antibody heavy chain gene, a human antibody light chain gene, and a human antibody surrogate light chain gene.
1 . A human artificial chromosome vector comprising a human antibody heavy chain gene, a human antibody light chain gene, and a human antibody surrogate light chain gene.
2 . The human artificial chromosome vector according to claim 1 , wherein the human antibody surrogate light chain gene is VpreB gene and λ5 gene.
3 . The human artificial chromosome vector according to claim 1 , comprising a gene of a non-human animal-derived IgM heavy chain constant region.
4 . The human artificial chromosome vector according to claim 3 , wherein the gene of the non-human animal-derived IgM heavy chain constant region is derived from bovine IGHM.
5 . An animal having the human artificial chromosome vector according to claim 1 .
6 . A bovine having the human artificial chromosome vector according to claim 3 .
7 . A method for producing a human antibody, comprising:
administering a target antigen into an animal according to claim 5 to produce and accumulate the human antibody specific to the antigen in serum of the animal, and recovering the human antibody specific to the antigen from the serum.
8 . A method for producing a human antibody, comprising:
administering a target antigen into a bovine according to claim 6 to produce and accumulate the human antibody in serum of the bovine, and recovering the human antibody specific to the antigen from the serum.