DEVICES FOR DETECTION OF ANALYTES
The invention provides “molecular nets” which may be used in diagnostic and other applications to detect analytes in a sample. A molecular net is a branched pseudorandom copolymer comprising two broad classes of subunits: capture agents and linking agents. The subunits self-assemble to form a structure capable of binding to predetermined targets. The binding can then be detected.
1 . An article of manufacture comprising
a first portion having a capture agent composition consisting one or more species of first capture agents and one or more species of first linking agents, wherein each capture agent in the first portion is connected by a first linking agent to at least one other capture agent in the first portion;
a second portion having a capture agent composition consisting of a one or more species of second capture agents, wherein each capture agent in the second portion is connected by a linking agent to at least one other capture agent in the second portion;
wherein at least one species of first capture agents differs from at least one species of second capture agents; and
wherein some capture agents in the first portion are connected by linking agents to capture agents in the second portion, such that the first portion and the second portion form a continuous molecular net.
2 . An article of manufacture comprising
a first portion having a capture agent composition consisting of a first plurality of heterogeneous capture agents and a linking agent composition consisting of a first plurality of heterogeneous linking agents, wherein each capture agent in the first portion is connected by a linking agent to at least one other capture agent in the first portion;
a second portion having a capture agent composition consisting of a second plurality of heterogeneous capture agents and a linking agent composition consisting of a second plurality of heterogeneous linking agents, wherein each capture agent in the second portion is connected by a linking agent to at least one other capture agent in the second portion;
wherein the capture agent composition and linking agent composition of the first portion differs from the capture agent composition and linking agent composition of the second portion; and
wherein at least some capture agent molecules in the first portion are connected by linking agents to at least some capture agent molecules in the second portion, such that the first portion and the second portion form a continuous molecular net.
3 . A branched pseudorandom copolymer,
wherein the monomers are capture agents and linking agents, the capture agents comprise a plurality of species of capture agents specific for different targets and the linking agents comprise a plurality of species of linking agents,
wherein the co-polymer is formed under conditions under which capture agent monomers are crosslinked to each other by linking agents.
4 . The copolymer of claim 3 wherein the linking agents are homobifunctional or heterobifunctional linkers and wherein some species of linking agents comprise one or more reactive groups that do not bind to some species of capture agents in the copolymer.
5 - 41 . (canceled)