Nucleic acid molecule having binding affinity to rodent-derived IgG antibody, binder, detection reagent, and detection kit
View Patent ↗The invention provides a nucleic acid molecule having a binding affinity to a rodent-derived IgG antibody, which can be prepared easier than an antibody and has a binding affinity equivalent or superior to that of an antibody, a binder using the nucleic acid molecule, a detection reagent, and a detection kit. The nucleic acid molecule of the invention has a binding affinity to a rodent-derived IgG antibody and has a dissociation constant of 1 μM or less. The binder for a rodent-derived IgG antibody of the present invention includes the nucleic acid molecule of the present invention. The detection reagent for detecting a rodent-derived IgG antibody of the invention includes the binder for a rodent-derived IgG antibody of the invention. The detection kit for detecting a rodent-derived IgG antibody of the invention includes the detection reagent for detecting a rodent-derived IgG antibody of the invention.
1. An isolated single-stranded nucleic acid molecule having a specific binding affinity to a mouse-derived IgG antibody, wherein said isolated single-stranded nucleic acid molecule comprises a nucleotide sequence selected from the group consisting of SEQ ID NOs: 1-7.
2. The isolated single-stranded nucleic acid molecule according to claim 1 , wherein the mouse-derived IgG antibody is at least one of a subtype 1 IgG antibody, a subtype 2a IgG antibody, and a subtype 3 IgG antibody.
3. A binder for a mouse-derived IgG antibody, comprising the isolated single-stranded nucleic acid molecule according to claim 1 .
4. A detection reagent for detecting a mouse-derived IgG antibody, comprising the binder according to claim 3 .
5. A detection kit for detecting a mouse-derived IgG antibody, comprising the detection reagent according to claim 4 .
6. A detection method comprising the following steps:
an immunoprecipitation step of reacting an antibody with an object to be detected in a sample as an antigen to immunoprecipitate;
a separation step of denaturing the immunoprecipitate to separate it from other components in the sample;
a first reaction step of reacting an antibody with the antigen of the immunoprecipitate separated in the separation step; and
a second reaction step of reacting a binder that specifically binds to the antibody in the first reaction step,
wherein the binder according to claim 3 is used as the binder in the second reaction step.
7. The detection method according to claim 6 , wherein SDS-PAGE is employed for the separation step and a Western blot analysis is employed for the first reaction step and the second reaction step.
8. The isolated single-stranded nucleic acid molecule according to claim 1 , wherein said isolated single-stranded nucleic acid molecule comprises the nucleotide sequence of SEQ ID NO: 1.
9. The isolated single-stranded nucleic acid molecule according to claim 1 , wherein said isolated single-stranded nucleic acid molecule comprises the nucleotide sequence of SEQ ID NO: 2.
10. The isolated single-stranded nucleic acid molecule according to claim 1 , wherein said isolated single-stranded nucleic acid molecule comprises the nucleotide sequence of SEQ ID NO: 3.
11. The isolated single-stranded nucleic acid molecule according to claim 1 , wherein said isolated single-stranded nucleic acid molecule comprises the nucleotide sequence of SEQ ID NO: 4.
12. The isolated single-stranded nucleic acid molecule according to claim 1 , wherein said isolated single-stranded nucleic acid molecule comprises the nucleotide sequence of SEQ ID NO: 5.
13. The isolated single-stranded nucleic acid molecule according to claim 1 , wherein said isolated single-stranded nucleic acid molecule comprises the nucleotide sequence of SEQ ID NO: 6.
14. The isolated single-stranded nucleic acid molecule according to claim 1 , wherein said isolated single-stranded nucleic acid molecule comprises the nucleotide sequence of SEQ ID NO: 7.