Decellularized adipose cell growth scaffold
Provided herein are methods of making a cell growth scaffold from adipose tissue, cell growth scaffolds having low lipid content and methods of using the cell growth scaffold.
1. A method of preparing an adipose tissue-derived cell growth scaffold comprising, in order: digesting an adipose tissue sample with a proteinase and a chelating agent; washing the adipose tissue sample with an emulsifier; disinfecting the adipose tissue sample; depyrogenating the adipose tissue sample; drying the adipose tissue sample; and washing the adipose tissue sample with n-propanol, isopropanol or a mixture thereof.
2. The method of claim 1 , wherein the method comprises the following steps, in order:
a. rinsing an adipose tissue sample;
b. mechanically massaging the adipose tissue sample;
c. digesting the adipose tissue sample with a proteinase and a chelating agent;
d. rinsing the adipose tissue sample;
e. mechanically massaging the tissue sample;
f. washing the adipose tissue sample with a surfactant while the adipose tissue sample is agitated;
g. rinsing the adipose tissue sample;
h. washing the adipose tissue sample with an emulsifier;
i. rinsing the adipose tissue sample;
j. disinfecting the adipose tissue sample;
k. depyrogenating the adipose tissue sample;
l. rinsing the adipose tissue sample;
m. drying the adipose tissue sample;
n. washing the dried adipose tissue sample in n-propanol, isopropanol or a mixture thereof;
o. washing the adipose tissue sample in an aqueous solvent; and
p. drying the adipose tissue sample.
3. The method of claim 2 , in which in step m. the sample is dried by lyophilization.
4. The method of claim 2 , in which step p. drying the sample comprises lyophilizing the sample.
5. The method of claim 2 , further comprising step q. comminuting the sample to produce a powder.
6. The method of claim 5 , wherein the powder has a maximum size of 250 μM.
7. The method of claim 1 , in which the adipose tissue sample is washed with n-propanol.
8. The method of claim 1 , in which the adipose tissue sample is washed with isopropanol.
9. The method of claim 1 , further comprising after washing the adipose tissue sample with n-propanol, isopropanol or a mixture thereof, placing the adipose tissue sample in a covering prepared from one or more sheets of ECM material.