Combined use of CRY1Ca and CRY1Fa proteins for insect resistance management
View Patent ↗The subject invention includes methods and plants for controlling lepidopteran insects. The plants comprise a Cry1Fa insecticidal protein and a Cry1Ca insecticidal protein, in combination, to delay or prevent development of resistance by the insect(s).
1. A transgenic plant comprising a DNA encoding the Cry1Ca insecticidal protein of SEQ ID NO:5 and a DNA encoding the Cry1Fa insecticidal protein of SEQ ID NO:4.
2. A seed of the plant of claim 1 wherein the seed comprises said DNA encoding said Cry1Ca insecticidal protein and said DNA encoding said Cry1Fa insecticidal protein.
3. A population of plants comprising non-Bt refuge plants and a plurality of the transgenic plants of claim 1 , wherein said refuge plants comprise less than 40% of said population of plants.
4. The population of plants of claim 3 , wherein said refuge plants comprise less than 5% of said population of plants.
5. A mixture of seeds comprising refuge seeds from non-Bt refuge plants, and a plurality of seeds of claim 2 , wherein said refuge seeds comprise less than 40% of all the seeds in the mixture.
6. The mixture of seeds of claim 5 , wherein said refuge seeds comprise less than 5% of all the seeds in the mixture.
7. The transgenic plant of claim 1 , said plant further comprising DNA encoding a Cry1Ab core toxin-containing protein.
8. A of plants comprising non-Bt refuge plants and a plurality of transgenic plants of claim 7 , wherein said refuge plants comprise less than about 20% of said population of plants.
9. The population of plants of claim 8 , wherein said refuge plants comprise less than about 10% of said population of plants.
10. A composition for controlling or preventing Cry-resistant Spodoptera frugiperda insects, said composition comprising cells that express insecticidally c active amounts of both the Cry1Fa core toxin-containing protein of SEQ ID NO: 4 and the Cry1Ca core toxin-containing protein of SEQ ID NO:5.
11. The composition of claim 10 , wherein said cell is a microorganism or a plant cell.
12. The transgenic plant of claim 1 , wherein said plant a third protein selected from the group consisting of Vip3A, Cry1D, Cry1Be, and Cry1E proteins.
13. A population of plants comprising non-Bt refuge plants and a plurality of transgenic plants of claim 12 , wherein said refuge plants comprise less than about 10% said population to plants.
14. The population of plants of claim 13 , wherein said refuge plants comprise less than about 5% of said population of plants.
15. A mixture of seeds comprising refuge seeds from non-Bt refuge plants and a plurality of seeds from the plant of claim 12 , wherein said refuge seeds comprise less than 10% of all the seeds in the mixture.
16. The plant of claim 1 , wherein said plant is selected from the group consisting of corn, soybeans, and cotton.
17. The plant of claim 16 , wherein said plant is a corn plant.