Combined use of Cry1Ca and Cry1Ab proteins for insect resistance management
View Patent ↗The subject invention includes methods and plants for controlling lepidopteran insects, said plants comprising Cry1Ca insecticidal protein and a Cry1Ab insecticidal protein in combination to delay or prevent development of resistance by the insects.
1. A transgenic plant comprising DNA encoding a Cry1C insecticidal protein comprising SEQ ID NO:2 and DNA encoding a Cry1Ab insecticidal protein comprising SEQ ID NO:3, wherein said transgenic plant is selected from the group consisting of corn, soybeans, and sugarcane.
2. Seed or part of the plant of claim 1 , wherein said seed or part comprises said DNA encoding said Cry1C insecticidal protein and DNA encoding said Cry1Ab insecticidal protein.
3. A population of plants rising transgenic plants of claim 1 and non-Bt refuge plants, wherein said population of plants is planted in a field and wherein said refuge plants comprise less than 40% of all crop plants in said field.
4. The population of plants of claim 3 , wherein said refuge plants comprise less than 5% of all crop plants in said field.
5. The population of plants of claim 3 , wherein said refuge plants are in blocks or strips.
6. A mixture of seeds comprising refuge seeds from non-Bt refuge plants, and a plurality of seeds of claim 2 , wherein said refuge seeds comprise less than 40% of all the seeds in the mixture.
7. The mixture of seeds of claim 6 , wherein said refuge seeds comprise less than 5% of all the seeds in the mixture.
8. A method of managing development of resistance to a Cry toxin by Spodoptera frugiperda insects, said method comprising planting seeds to produce the population of plants of claim 3 .
9. The plant of claim 1 , said plant further comprising DNA encoding a Cry1Fa core toxin-containing protein.
10. A population of plants comprising transgenic plants of claim 9 and non-Bt refuge plants, wherein said population of plants is planted in a field and wherein said refuge plants comprise less than about 20% of all crop plants in said field.
11. The population of plants of claim 10 , wherein said field comprises less than about 10% refuge plants.
12. A method of managing development of resistance to a Cry toxin by Spodoptera frugiperda insects, said method comprising planting seeds to produce the population field of plants of claim 11 .
13. A composition for controlling Spodoptera frugiperda insects, said composition comprising cells that express effective amounts of both a Cry1Ab core toxin-containing protein comprising SEQ ID NO:3 and a Cry1C core toxin-containing protein comprising SEQ ID NO:2.
14. The composition of claim 13 comprising a host transformed to express both the Cry1Ab core toxin-containing protein and the Cry1C core toxin containing protein, wherein said host is a microorganism or a plant cell.
15. A method of controlling Spodoptera frugiperda insects, said method comprising presenting to said insects or to the environment of said insects an effective amount of the composition of claim 13 .
16. The plant of claim 1 , wherein said plant is a maize plant.
17. A plant cell of the plant of claim 1 , wherein said plant cell comprises said DNA encoding said Cry1C insecticidal protein and said DNA encoding said Cry1Ab insecticidal protein, wherein said Cry1C insecticidal protein comprises SEQ ID NO:2 and said Cry1Ab insecticidal protein comprises SEQ ID NO:3.
18. A plant cell of the plant of claim 9 , wherein said plant cell comprises said DNA encoding said Cry1C insecticidal protein and said DNA encoding said Cry1Ab insecticidal protein, wherein said Cry1C insecticidal protein comprises SEQ ID NO:2 and said Cry1Ab insecticidal protein comprises SEQ ID NO:3.
19. A plant cell of the plant of claim 16 , wherein said plant cell comprises said DNA encoding said Cry1C insecticidal protein and said DNA encoding said Cry1Ab insecticidal protein, wherein said Cry1C insecticidal protein comprises SEQ ID NO:2 and said Cry1Ab insecticidal protein comprises SEQ ID NO:3.