Use of Cry1Da in combination with Cry1Be for management of resistant insects
View Patent ↗The subject invention includes methods and plants for controlling fall army worm insects, said plants comprising a Cry1Da insecticidal protein and a Cry1Be insecticidal protein, and various combinations of other proteins comprising this pair of proteins, to delay or prevent development of resistance by the insects.
1. A transgenic corn or soybean plant comprising DNA encoding the Cry1Da of SEQ ID NO:2, DNA encoding Cry1Be of SEQ ID NO:1, and DNA encoding Cry1Fa, and wherein said transgenic plant expresses said Cry1Be, said Cry1Da, and said Cry1Fa in amounts that are insecticidally active against a Cry resistant fall armyworm.
2. A seed of the plant of claim 1 , wherein said seed comprises the DNA encoding Cry1Da, DNA encoding Cry1Be, and the DNA encoding Cry1Fa.
3. A plurality of corn or soybean plants, comprising non-Bt refuge plants and a plurality of transgenic plants of claim 1 , wherein said refuge plants comprise less than 40% of all crop plants in said plurality of plants, and wherein said plurality of plants comprises at least one refuge plant.
4. The plurality of plants of claim 3 , wherein said refuge plants comprise less than 30% of all the crop plants in said plurality of plants.
5. The plurality of plants of claim 3 , wherein said refuge plants comprise less than 20% of all the crop plants in said plurality of plants.
6. The plurality of plants of claim 3 , wherein said refuge plants comprise less than 10% of all the crop plants in said plurality of plants.
7. The plurality of plants of claim 3 , wherein said refuge plants comprise less than 5% of all the crop plants in said plurality of plants.
8. A mixture of seeds comprising refuge seeds from non-Bt refuge plants, and a plurality of seeds of claim 2 , wherein said refuge seeds comprise less than 40% of all the seeds in the mixture.
9. The mixture of seeds of claim 8 , wherein said refuge seeds comprise less than 30% of all the seeds in the mixture.
10. The mixture of seeds of claim 8 , wherein said refuge seeds comprise less than 20% of all the seeds in the mixture.
11. The mixture of seeds of claim 8 , wherein said refuge seeds comprise less than 10% of all the seeds in the mixture.
12. The mixture of seeds of claim 8 , wherein said refuge seeds comprise less than 5% of all the seeds in the mixture.
13. A method of managing development of resistance to a Cry toxin by a fall armyworm, said method comprising planting the mixture of seeds of claim 8 .
14. The plant of claim 1 , wherein said plant is a maize plant.
15. A method of controlling a Cry protein resistant fall armyworm insect, said method comprising planting the mixture of seeds of claim 8 .