Process for production of L-glutamine or L-glutamic acid
View Patent ↗Provided is an efficient process for producing L-glutamine or L-glutamic acid using a microorganism. L-glutamine or L-glutamic acid is produced by culturing in a medium a microorganism in which has an ability to produce L-glutamine or L-glutamic acid, and in which an ability to form a superhelical double-stranded DNA is decreased compared with that of the parent strain, producing and accumulating L-glutamine or L-glutamic acid in the medium, and recovering L-glutamine or L-glutamic acid from the medium.
1. A process for producing L-glutamine or L-glutamic acid which comprises:
(a) culturing in a medium bacteria, which have an ability to produce L-glutamine or L-glutamic acid, in which an ability to form a superhelical double-stranded DNA is decreased compared with that of the parent strain, and in which the production of L-glutamine or L-glutamic acid is increased compared with that of the parent strain,
(b) producing and accumulating L-glutamine or L-glutamic acid in the medium, and
(c) recovering L-glutamine or L-glutamic acid from the medium.
2. The process for producing L-glutamine or L-glutamic acid according to claim 1 , wherein the bacteria are the bacteria in which DNA gyrase activity is decreased compared with that of the parent strain.
3. The process for producing L-glutamine or L-glutamic acid according to claim 2 , wherein the bacteria in which DNA gyrase activity is decreased compared with that of the parent strain is the bacteria in which an activity of a DNA gyrase inhibitory protein is increased compared with that of the parent strain.
4. The process for producing L-glutamine or L-glutamic acid according to claim 1 , wherein the bacteria are the bacteria in which topoisomerase activity is increased compared with that of the parent strain.