Process for production of L-amino acid
View Patent ↗Provided is a novel process for producing an L-amino acid using a microorganism belonging to the genus Escherichia . According to the present invention, a process for producing an L-amino acid comprising; culturing a microorganism in which an activity of the protein of any of the following [1]-[3] is increased compared with that of the parent strain in a medium, producing and accumulating the L-amino acid in the medium, and recovering the L-amino acid from the medium: [1] a protein comprising the amino acid sequence shown in SEQ ID NO: 2 [2] a protein consisting of an amino acid sequence in which one or more amino acids are deleted, substituted or added in the amino acid sequence shown in SEQ ID NO: 2, and having YeiG activity [3] a protein consisting of an amino acid sequence having 80% or more homology to the amino acid sequence shown in SEQ ID NO: 2, and having YeiG activity.
1. A process for producing an L-amino acid comprising:
culturing a microorganism in which an activity of a protein is increased compared to the parent strain in a medium,
producing and accumulating the L-amino acid in the medium, and
recovering the L-amino acid from the medium,
wherein the protein is
[1] a protein comprising the amino acid sequence shown in SEQ ID NO:2, or
[2] a homologous protein consisting of an amino acid sequence having 95% or more identity to the amino acid sequence shown in SEQ ID NO: 2, and having YeiG activity.
2. The process for producing the L-amino acid according to claim 1 , wherein the microorganism is transformed with a DNA, or the microorganism has increased expression of the gene encoding the protein compared to the parent strain by modifying an expression regulatory sequence of the DNA, wherein the DNA is
[1] a DNA encoding the protein,
[2] a DNA comprising the nucleotide sequence shown in SEQ ID NO: 1, or
[3] a DNA which hybridizes to the DNA consisting of a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 1 under stringent conditions, and encodes a homologous protein having YeiG activity, wherein the stringent conditions including incubating a DNA-immobilized filter and a probe DNA in a solution containing 50% formamide, 5×SSC (750 mmol/l sodium chloride, 75 mmol/l sodium citrate), 50 mmol/l sodium phosphate (pH 7.6), 5×Denhardt's solution, 10% dextran sulfate and 20 μg/l dentured salmon sperm DNA at 42° C. overnight and washing the filter with 0.2×SSC solution at about 65° C.
3. The process for producing the L-amino acid according to claim 1 , wherein the microorganism belongs to the genus Escherichia.
4. The process for producing the L-amino acid according to claim 3 , wherein the L-amino acid is L-glutamine.
5. The process for producing the L-amino acid according to claim 2 , wherein the L-amino acid is L-glutamine.
6. The process for producing the L-amino acid according to claim 2 , wherein the microorganism belongs to the genus Escherichia.
7. The process for producing the L-amino acid according to claim 6 , wherein the L-amino acid is L-glutamine.
8. The process for producing the L-amino acid according to claim 1 , wherein the L-amino acid is L-glutamine.
9. The process for producing the L-amino acid according to claim 1 , wherein the protein is a protein comprising the amino acid sequence shown in SEQ ID NO: 2.
10. The process for producing the L-amino acid according to claim 1 , wherein the protein is a homologous protein consisting of an amino acid sequence having 95% or more identity to the amino acid sequence shown in SEQ ID NO: 2, and having YeiG activity.
11. The process for producing the L-amino acid according to claim 2 , wherein the DNA is a DNA encoding the protein.
12. The process for producing the L-amino acid according to claim 2 , wherein the DNA is a DNA comprising the nucleotide sequence shown in SEQ ID NO: 1.
13. The process for producing the L-amino acid according to claim 2 , wherein the DNA is a DNA which hybridizes to the DNA consisting of a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 1 under stringent conditions, and encodes a homologous protein having YeiG activity, wherein the stringent conditions include incubating a DNA-immobilized filter and a probe DNA in a solution containing 50% formamide, 5×SSC (750 mmol/l sodium chloride, 75 mmol/l sodium citrate), 50 mmol/l sodium phosphate (pH 7.6), 5×Denhardt's solution, 10% dextran sulfate and 20 μg/l denatured salmon sperm DNA at 42° C. overnight and washing the filter with 0.2×SSC solution at about 65° C.