Method of cancer treatment
A method and composition for treating cancer comprising administering to a patient an effective amount of attenuated Salmonella typhimurium containing a plasmid carrying the coding sequence encoding a truncated human interleukin-2 and optionally an oil containing a high antioxidant concentration.
1. An anti-tumor agent comprising an effective amount of attenuated Salmonella typhimurium containing a plasmid carrying a coding sequence encoding a truncated human interleukin-2, wherein the truncated human interleukin-2 consists of the amino acid sequence shown in SEQ ID NO:2.
2. The anti-tumor agent of claim 1 wherein the plasmid comprises SEQ ID NO:5.
3. The anti-tumor agent of claim 1 wherein the plasmid comprises SEQ ID NO:1.
4. An anti-tumor agent comprising an affective amount of attenuated Salmonella typhimurium containing a coding sequence encoding the truncated human interleukin-2 of SEQ ID NO:2, wherein the coding sequence has 95-100% identity to SEQ ID NO:1.
5. The anti-tumor agent of claims 1 , 2 , 3 or 4 wherein the attenuated Salmonella typhimurium containing the coding sequence encoding the truncated human interleukin-2 lacks the cyclic AMP and cAMP receptor protein.
6. The anti-tumor agent of claim 5 wherein the attenuated Salmonella typhimurium lacks DNA encoding enzyme aspartate semialdehyde dehydrogenase and the plasmid encodes said dehydrogenase and wherein the plasmid also encodes said truncated human interleukin-2.