Method for enhancing immune response in the treatment of infectious and malignant diseases
The present invention pertains to a new approach for the treatment of infectious and malignant diseases. The present invention provides new DNA and protein vaccines for the treatment of infectious and malignant diseases through enhancing immune response.
1. A method for enhancing immune response in a subject under the treatment of an infectious or malignant disease, comprising administering the subject topically, subcutaneously or intramuscularly with a pharmaceutical composition comprising a DNA construct fused with a mammalian expression vector and a pharmaceutically acceptable carrier, wherein the DNA construct comprise a polynucleotide sequence encoding CTLA-4 or a fragment thereof, PD-1 or a fragment thereof, and PD-L1 or a fragment thereof.
2. The method of claim 1 , wherein the subject is treated with an anti-infection or anti-cancer drug causing a stimulation of immune response in the subject.
3. The method of claim 1 , wherein the subject is under the treatment of a malignant disease.
4. The method of any one of claims 1 - 3 , wherein the malignant disease is selected from the group consisting of metastatic melanoma, pancreatic cancer, colorectal cancer, hepatocellular carcinoma, lymphoma, hormone refractory prostate cancer, ovarian cancer, acute myeloid leukemia, and non-small cell lung cancer.
5. The method of claim 1 , wherein the pharmaceutical composition is administered subcutaneously.
6. The method of claim 1 , wherein the pharmaceutical composition is administered intramuscularly.