Kit for measuring the thrombin generation in a sample of a patient's blood or plasma
View Patent ↗The invention provides a kit for measuring the thrombin generation in a sample of a patient's blood or plasma, or in a sample of clotting factors. The kit contains lyophilized tissue factor/phospholipid-complex and a lyophilized mixture containing a thrombin-substrate and CaCl 2 . The invention also provides processes for preparing the reagents for the kit. The kit can be used in a method for measuring the thrombin generation in a sample, wherein it is possible to detect changes in thrombin generation kinetics, for example after administration of inhibitor bypassing agents to a patient who has developed inhibitors to an exogenous clotting factor such as Factor VIII.
1. A method for measuring the thrombin generation in whole blood or plasma, comprising the steps of:
(a) providing a lyophilized tissue factor (TF)/phospholipid (PL)-complex and a lyophilized mixture that is dissolvable in water and contains a thrombin-substrate comprising a fluorescent label and CaCl 2 ;
(b) mixing said lyophilized TF/PL-complex and said lyophilized mixture containing thrombin-substrate and CaCl 2 in an amount of plasma or whole blood sample that provides a concentration of 1 mM thrombin substrate and 15 mM CaCl 2 , wherein the lyophilized TF/PL-complex and the lyophilized mixture containing the thrombin-substrate and CaCl 2 are immobilized on a solid support; and
(c) measuring the thrombin generation in said sample.
2. The method according to claim 1 , wherein the sample is a cell-free plasma sample.
3. The method of claim 1 , wherein the thrombin substrate is Z-Gly-Gly-Arg-AMC.
4. The method of claim 1 , wherein the concentration of TF in the lyophilized TF/PL-complex ranges from about 5 to about 1000 pM.
5. The method of claim 1 , wherein the concentration of PL in the lyophilized TF/PL-complex ranges from about 1 to about 100 μM.
6. The method of claim 1 , wherein the TF is of recombinant origin.
7. The method of claim 1 , wherein the PL is a mixture comprising phosphatidylserine (PS) and phosphatidylcholine (PC); or a mixture comprising PS, PC, and phosphatidylethanolamine (PE).
8. The method of claim 1 , wherein the PL is a mixture of PS and PC, and the weight ratio of PC/PS is in the range of from about 60/40 to about 95/5 based on the total amount of phospholipid.
9. The method of claim 1 , wherein the PL is a mixture of PS, PC, and PE, and the weight ratio of PC/PS/PE is in the range of from about 60/20/20 to about 78/17/5, based on the total amount of phospholipids.
10. The method of claim 1 , wherein the solid support is the inner surface of a well of a microtiter plate.