Modified ethylenediamine-N, N′-disuccinate: ethylenediamine lyase
View Patent ↗The present invention provides a modified ethylenediamine-N,N′-disuccinate:ethylenediamine lyase. The present invention also provides a protein that comprises the amino acid sequence represented by SEQ ID NO: 1; or a protein that comprises an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO: 1 by deletion, substitution, or addition of one or more amino acid residues, and has an ethylenediamine-N,N′-disuccinate:ethylenediamine lyase activity.
1. A recombinant vector comprising an isolated gene encoding a protein comprising the amino acid sequence of SEQ ID NO:1 and having an ethylenediamine-N,N′-disuccinate:ethylenediamine lyase activity.
2. An isolated gene encoding a modified protein comprising the amino acid sequence of SEQ ID NO:1 having 1 to 10 substitutions, and wherein the protein has ethylenediamine-N,N′-disuccinate:ethylenediamine lyase activity that is heat resistant.
3. The isolated gene of claim 2 , wherein one substitution is the substitution of the lysine residue at position 120 with a glutamic acid residue.
4. The isolated gene of claim 2 , wherein one substitution is the substitution of the isoleucine residue at position 166 with a serine residue.
5. The isolated gene of claim 2 , wherein one substitution is the substitution of the isoleucine residue at position 166 with a threonine residue.
6. The isolated gene of claim 2 , wherein one substitution is the substitution of the alanine residue at position 365 with a valine residue.
7. An isolated gene encoding a modified protein comprising the amino acid sequence of SEQ ID NO:1 having 2 to 10 substitutions, wherein the substitutions include the substitution of the lysine residue at position 120 with a glutamic acid residue, and wherein the protein has ethylenediamine-N,N′-disuccinate:ethylenediamine lyase activity that is heat resistant.
8. The isolated gene of claim 7 , wherein the isoleucine residue at position 166 is substituted with a serine residue.
9. The isolated gene of claim 7 , wherein the isoleucine residue at position 166 is substituted with a threonine residue.
10. An isolated gene encoding a modified protein comprising the amino acid sequence of SEQ ID NO:1 having 2 to 10 substitutions, wherein the substitutions include the substitution of the isoleucine residue at position 166 with a serine residue, and the substitution of the alanine residue at position 365 with a valine residue, and wherein the protein has ethylenediamine-N,N′-disuccinate:ethylenediamine lyase activity that is heat resistant.
11. An isolated gene encoding a modified protein comprising the amino acid sequence of SEQ ID NO:1 having 2 to 10 substitutions, wherein the substitutions include the substitution of the isoleucine residue at position 166 with a threonine residue, and the substitution of the alanine residue at position 365 with a valine residue, and wherein the protein has ethylenediamine-N,N′-disuccinate:ethylenediamine lyase activity that is heat resistant.
12. An isolated gene encoding a modified protein comprising the amino acid sequence of SEQ ID NO:1 having 3 to 10 substitutions, wherein the substitutions include the substitution of the lysine residue at position 120 with a glutamic acid residue, and the substitution of the isoleucine residue at position 166 with a serine residue and the substitution of the alanine residue at position 365 with a valine residue, and wherein the protein has ethylenediamine-N,N′-disuccinate:ethylenediamine lyase activity that is heat resistant.
13. An isolated gene encoding a modified protein comprising the amino acid sequence of SEQ ID NO:1 having 3 to 10 substitutions, wherein the substitutions include the substitution of the lysine residue at position 120 with a glutamic acid residue, and the substitution of the isoleucine residue at position 166 with a threonine residue and the substitution of the alanine residue at position 365 with a valine residue, and wherein the protein has ethylenediamine-N,N′-disuccinate:ethylenediamine lyase activity that is heat resistant.