Methods of determining the presence and/or amount of a biomarker to determine the presence, identity, and/or severity of a lysosomal storage or neurological disorder
View Patent ↗Provided herein are methods of diagnosing or monitoring the treatment of abnormal glycan accumulation or a disorder associated with abnormal glycan accumulation.
1. A method of determining the presence of and/or measuring the amount of a biomarker to determine the presence, identity, and/or severity of the disease or condition in an individual having a disease or condition associated with abnormal glycan biosynthesis, degradation, or accumulation, the method comprising:
(a) generating the biomarker comprising of one or more non-reducing end glycan residual compound(s), wherein the biomarker is generated by treating a population of glycans, in or isolated from a biological sample from the individual, with at least one digesting glycan enzyme(s), wherein prior to enzyme treatment, the biomarker is not present in abundance in samples from individuals with the disease or condition relative to individuals without the disease or condition, and
(b) using an analytical instrument to detect the presence of and/or measure the amount of the biomarker produced and displaying or recording the presence of or a measure of a population of the biomarker; and
(c) determining the presence, identity, and/or severity of the disease or condition utilizing the presence of and/or measure the amount of the biomarker;
wherein the disease or condition is a lysosomal storage disorder or a neurological disorder; and
wherein when the lysosomal storage disorder is an MPS disorder, then the digesting glycan enzyme is not a lyase.
2. The method of claim 1 , wherein the disease or disorder is caused by an abnormally functioning glycan degradation enzyme and wherein the abnormally functioning glycan degradation enzyme and the normally functioning glycan degradation enzyme are of the same type.
3. The method of claim 1 , wherein the abnormal glycan accumulation comprises the accumulation of abnormal amounts of normal glycans.
4. The method of claim 1 , wherein the abnormal glycan accumulation comprises the accumulation of abnormal amounts of abnormal glycans.
5. The method of claim 1 , wherein the normally functioning glycan degradation enzyme is a glycosidase, sulfatase, phosphorylase, deacetylase, or a combination thereof.
6. The method of claim 5 , wherein the normally functioning glycan degradation enzyme is a glycosidase selected from an exo-glycosidase and an endo-glycosidase.
7. The method of claim 6 wherein the glycosidase is an exo-glycosidase selected from the group consisting of a galactosidase, and a glucuronidase.
8. The method of claim 6 , wherein the glycan residual compound is a monosaccharide.
9. The method of claim 5 , wherein the glycan residual compound is sulfate, phosphate, acetate, or a combination thereof.
10. The method of claim 1 , further comprising purifying a biological sample prior to transforming a glycan thereof wherein the process of purifying a biological sample comprises removing monosaccharides therefrom, removing sulfates therefrom, removing phosphates therefrom, removing acetate therefrom, or a combination thereof.
11. The method of claim 1 , wherein transforming a glycan of a biological sample with a normally functioning glycan degradation enzyme comprises transforming a glycan of a biological sample with a plurality of normally functioning glycan degradation enzymes concurrently, sequentially, or a combination thereof.
12. The method of claim 1 , wherein the disorder associated with an abnormal glycan accumulation is Alzheimer's disease, Parkinson's disease, MPS I, MPS II, MPS IIIA, MPS IVA, MPS VI, Gaucher disease, Krabbe disease, Pompe disease, Sandhoff disease, Tay-Sachs disease, or Fabry Disease.
13. The method of claim 1 , wherein determining whether the amount of liberated glycan residue is abnormal comprises labeling the glycan residue with a detectable label and measuring the amount of labeled glycan residue with an analytical instrument using UV-Vis spectroscopy, IR spectroscopy, mass spectrometry, or a combination thereof.
14. The method of claim 13 , wherein the detectable label is a mass label, a radioisotope label, a fluorescent label, a chromophore label, or affinity label.
15. The method of claim 1 , wherein prior to measuring the amount of a population of non-reducing end glycan residual compounds, the non-reducing end glycan residual compounds are labeled with a detectable label and wherein the amount of liberated glycan is measured using UV-Vis spectroscopy, IR spectroscopy, mass spectrometry, or a combination thereof.
16. The method of claim 15 , wherein the detectable label is a mass label, a radioisotope label, a fluorescent label, a chromophore label, or affinity label.
17. A method monitoring the treatment of a disorder associated with the abnormal degradation, biosynthesis and/or accumulation of glycans, the method comprising:
(a) following administration of an agent for treating a disorder associated with the abnormal degradation, biosynthesis and/or accumulation of glycans to an individual in need thereof, using an analytical instrument to measure the amount of a population of a biomarker comprising non-reducing end glycan residual compounds present in a transformed biological sample, the biomarker being generated by treating a population of glycans, in or isolated from a biological sample from the individual, with at least one digesting glycan enzyme(s), wherein prior to enzyme treatment, the biomarker is not present in abundance in samples from individuals with the disease or condition relative to individuals without the disease or condition, and
(b) determining whether or not the amount of biomarker has decreased or increased at a slower rate compared to the amount or rate of increase prior to administration of the agent for treating a disorder associated with the abnormal degradation, biosynthesis and/or accumulation of glycans; and
wherein the disorder is a lysosomal disorder or a neurological disorder.
18. The method of claim 17 , wherein the normally functioning glycan degradation enzyme is a glycosidase, sulfatase, phosphorylase, deacetylase, or a combination thereof.
19. The method of claim 18 , wherein the normally functioning glycan degradation enzyme is a glycosidase selected from an exo-glycosidase and an endo-glycosidase.
20. The method of claim 17 , wherein the glycan residual compound is a monosaccharide, sulfate, phosphate, acetate, or a combination thereof.
21. The method of claim 17 , wherein transforming a glycan of a biological sample with a normally functioning glycan degradation enzyme comprises transforming a glycan of a biological sample with a plurality of normally functioning glycan degradation enzymes concurrently, sequentially, or a combination thereof.