Fluidics devices

1. A fluidics device comprising:

a flow channel for transporting a fluid sample;

an inlet port for receiving said fluid sample, said inlet port in fluid communication with a first bridge valve;

a diaphragm configured and adapted to interface with a pump, said diaphragm being coupled to said first bridge valve;

a second bridge valve in fluid communication with said flow channel, said second valve being coupled to said diaphragm,

wherein said diaphragm and said bridge valves are integral to a common membranous layer in a multilayer assembly, and

wherein each of said first bridge valve and said second bridge valves comprises a displaceable region between substantially parallel slits in the membranous layer, wherein the displaceable region allows said fluid sample to flow under or over said displaceable region according to a direction of displacement thereof.

2. The fluidics device of claim 1 , further comprising a working electrode located in said flow channel.

3. The fluidics device of claim 2 , further comprising an auxiliary electrode.

4. The fluidics device of claim 1 , further comprising a connector configured and adapted to interface with a detection device.

5. The fluidics device of claim 1 , further comprising an Electrically Erasable-Programmable Read-Only Memory (EEPROM).

6. The fluidics device of claim 1 , further comprising a binding partner or a ligand immobilized in said flow channel.

7. The fluidics device of claim 6 , wherein said binding partner or ligand is immobilized using a self-assembling monolayer that is affixed to an electrode surface.

8. The fluidics device of claim 6 , wherein said binding partner or ligand is selected from the group consisting of a polynucleotide, a protein, a peptide, an antibody, and a combination thereof.

9. The fluidics device of claim 1 , further comprising an array of electrodes, each bound to a binding partner or a ligand.

10. The fluidics device of claim 9 , wherein said electrode array comprises a plurality of different binding partners and/or ligands immobilized thereon.

11. The fluidics device of claim 9 , wherein said electrode array is located in a serpentine channel.

12. The fluidics device of claim 1 , wherein said flow channel comprises a cross-sectional dimension comprising a greater width than height.

13. The fluidics device of claim 1 , having a sample capacity ranging from about 50 μL to about 200 μL.

14. The fluidics device of claim 9 , having a single cartridge capacity of no more than about 200 μL, wherein said array is contained in a flow channel having a length ranging from about 100 mm to about 200 mm and a cross-sectional dimension ranging from about 0.75 mm to about 2.0 mm in width and about 0.125 mm to about 0.40 mm in height.

15. A diagnostic kit comprising the fluidics device of claim 1 .

16. A method of determining analyte binding, comprising:

providing the fluidics device according to claim 9 ;

adding to said device the fluid sample, wherein the fluid sample is suspected of containing an analyte specific for said binding partner or ligand;

circulating and recirculating said fluid sample across said electrode array; and

detecting binding of said analyte to said binding partner or ligand.

17. The method of claim 16 , wherein said circulating and recirculating is performed at a rate ranging from about 10 μL/sec to about 40 μL/sec.