Methods and compositions for producing fatty alcohols and fatty aldehydes
View Patent ↗Compositions and methods for producing hydrocarbons such as aldehydes, alkanes, and alkenes are described herein. Certain hydrocarbons can be used in biofuels.
1. A method of producing a fatty aldehyde or a fatty alcohol, the method comprising:
(a) expressing a gene encoding a polypeptide that catalyzes the conversion of an acyl-ACP to a fatty aldehyde in an engineered microorganism;
(b) culturing said engineered microorganism in a culture media containing a carbohydrate carbon source under conditions effective to produce a fatty aldehyde or a fatty alcohol in the culture media, wherein said polypeptide comprises an amino acid sequence having at least 90% sequence identity to SEQ ID NO: 66.
2. The method of claim 1 , wherein said polypeptide comprises an amino acid sequence having at least 95% sequence identity to SEQ ID NO: 66.
3. The method of claim 1 , wherein said polypeptide has a higher affinity for acyl-ACP as compared to acyl-CoA.
4. The method of claim 1 , wherein the engineered microorganism is selected from the group consisting of a yeast, a fungi, a filamentous fungi, an algae and a bacteria.
5. The method of claim 1 , wherein the fatty aldehyde or fatty alcohol comprises a C 13 to C 21 fatty aldehyde or fatty alcohol.
6. A cell culture comprising a recombinant microorganism and a culture medium containing a carbon source, wherein a polypeptide that catalyzes the conversion of an acyl-ACP to a fatty aldehyde is overexpressed in said recombinant microorganism and a fatty aldehyde or a fatty alcohol is produced in the cell culture when said recombinant microorganism is cultured in the culture medium under conditions effective to express said polypeptide, wherein said polypeptide comprises an amino acid sequence having at least 90% sequence identity to SEQ ID NO: 66.
7. The cell culture of claim 6 , wherein said polypeptide comprises an amino acid sequence having at least 95% sequence identity to SEQ ID NO: 66.
8. The cell culture of claim 6 , wherein said polypeptide has a higher affinity for acyl-ACP as compared to acyl-CoA.
9. The cell culture of claim 6 , wherein the engineered microorganism is selected from the group consisting of a yeast, a fungi, a filamentous fungi, an algae and a bacteria.
10. The cell culture of claim 6 , wherein the fatty aldehyde or fatty alcohol comprises a C 13 to C 21 fatty aldehyde or fatty alcohol.
11. The cell culture of claim 6 , wherein the fatty aldehyde is selected from the group consisting of tetradecanal, hexadecanal, hexadecenal, octadecanal, octadecenal, methyltetradecanal, methyltetradecenal, methylhexadecanal, methylhexadecanal, methyloctadecanal, and methyloctadecenal.
12. The cell culture of claim 6 , comprising a fatty aldehyde or a fatty alcohol having a δ 13 C of about −15.4 or greater.
13. The cell culture of claim 6 , comprising a fatty aldehyde or a fatty alcohol having an f M 14 C of at least about 1.003.
14. A cell culture comprising a recombinant microorganism and culture medium containing a carbon source,
wherein a polypeptide that catalyzes the conversion of an acyl-ACP to a fatty aldehyde is overexpressed in said recombinant microorganism and a fatty aldehyde or a fatty alcohol is produced in the cell culture medium when said recombinant microorganism is cultured under conditions effective to express said polypeptide and wherein said polypeptide comprises amino acids 126-136 and 138-143 of SEQ ID NO: 66.
15. The cell culture of claim 14 , wherein the engineered microorganism is selected from the group consisting of a yeast, a fungi, a filamentous fungi, an algae and a bacteria.
16. The cell culture of claim 14 , wherein the fatty aldehyde or fatty alcohol comprises a C 13 to C 21 fatty aldehyde or fatty alcohol.
17. The cell culture of claim 14 , comprising a fatty aldehyde or a fatty alcohol having a δ 13 C of about −15.4 or greater.
18. The cell culture of claim 14 , comprising a fatty aldehyde or a fatty alcohol having an f M 14 C of at least about 1.003.
19. A cell culture comprising a recombinant microorganism and culture medium containing a carbon source, wherein a polypeptide that catalyzes the conversion of an acyl-ACP to a fatty aldehyde is overexpressed in said recombinant microorganism and a fatty aldehyde or a fatty alcohol is produced in the cell culture medium when said recombinant microorganism is cultured under conditions effective to express said polypeptide and wherein said polypeptide comprises amino acids 156-159 and 161-175 of SEQ ID NO: 66.
20. The cell culture of claim 19 , wherein the engineered microorganism is selected from the group consisting of a yeast, a fungi, a filamentous fungi, an algae and a bacteria.
21. The cell culture of claim 19 , wherein the fatty aldehyde or fatty alcohol comprises a C 13 to C 21 fatty aldehyde or fatty alcohol.
22. The cell culture of claim 19 , comprising a fatty aldehyde or a fatty alcohol having a δ 13 C of about −15.4 or greater.
23. The cell culture of claim 19 , comprising a fatty aldehyde or a fatty alcohol having an f M 14 C of at least about 1.003.
24. A cell culture comprising a recombinant microorganism and culture medium containing a carbon source, wherein a polypeptide that catalyzes the conversion of an acyl-ACP to a fatty aldehyde is overexpressed in said recombinant microorganism and a fatty aldehyde or a fatty alcohol is produced in the cell culture medium when said recombinant microorganism is cultured under conditions effective to express said polypeptide and wherein said polypeptide comprises amino acids 156-175 of SEQ ID NO: 66.
25. The cell culture of claim 24 , wherein the engineered microorganism is selected from the group consisting of a yeast, a fungi, a filamentous fungi, an algae and a bacteria.
26. The cell culture of claim 24 , wherein the fatty aldehyde or fatty alcohol comprises a C 13 to C 21 fatty aldehyde or fatty alcohol.