Fidgetin-like 2 as a target to enhance wound healing
View Patent ↗Methods of treating a wound in a subject are provided comprising administering to the subject an amount of an inhibitor of Fidgetin-like 2. Compositions and pharmaceutical compositions comprising an amount of an inhibitor of Fidgetin-like 2 are also provided. Methods are also provided for identifying an inhibitor of Fidgetin-like 2.
1. A method of treating a wound in a subject comprising directly administering to a wound of the subject an amount of a siRNA or shRNA directed against a DNA or RNA encoding human Fidgetin-like 2 comprising the amino acid sequence set forth in SEQ ID NO:2 effective to treat a wound in a subject.
2. The method of claim 1 , wherein the amount of the siRNA or shRNA directed against a DNA or RNA encoding human Fidgetin-like 2 is effective to accelerate wound healing.
3. The method of claim 1 , wherein the siRNA or shRNA directed against a DNA or RNA encoding human Fidgetin-like 2 is administered topically to the wound.
4. The method of claim 1 , wherein the siRNA is administered.
5. The method of claim 1 , wherein the siRNA or shRNA is directed against an mRNA encoding the human Fidgetin-like 2.
6. The method of claim 4 , wherein the siRNA comprises a sequence set forth in SEQ ID NOS:3, 4, 5, 6, 7, 8, 9, or 10.
7. The method of claim 1 , wherein the wound is an epidermal wound.
8. The method of claim 1 , wherein the wound is a skin wound.
9. The method of claim 1 , wherein the wound is a cardiac tissue wound.
10. The method of claim 9 , wherein the wound is a cardiovascular wound resulting from a myocardial infarction.
11. The method of claim 1 , wherein the wound is a central nervous system wound or peripheral nervous system wound.
12. The method of claim 4 , wherein the siRNA is encapsulated in a nanoparticle.
13. The method of claim 12 , wherein the nanoparticle is a liposomal nanoparticle.
14. A method of treating a neuronal wound in a subject comprising administering directly to the wound in the subject an amount of a siRNA or shRNA directed against a DNA or an RNA encoding human Fidgetin encoded by the nucleic acid sequence set forth in SEQ ID NO:12 effective to treat a neuronal wound in a subject.
15. The method of claim 4 , wherein the siRNA directed against a DNA or RNA encoding human Fidgetin-like 2 has at least one 2′ sugar modification.