Manipulating droplet size
View Patent ↗The invention generally relates to methods and systems for manipulating droplet size. In certain aspects, the invention provides methods for manipulating droplet size that include forming droplets of aqueous fluid surrounded by an immiscible carrier fluid, and manipulating droplet size during the forming step by adjusting pressure exerted on the aqueous fluid or the carrier fluid.
1. A method for droplet formation, the method comprising the steps of:
providing a plurality of aqueous fluids each in its own aqueous fluid channel in fluid communication with one or more immiscible carrier fluid channels;
forming droplets of aqueous fluid surrounded by an immiscible carrier fluid in the aqueous fluid channels;
applying a same constant pressure to the carrier fluid in each of the immiscible carrier fluid channels; and
adjusting pressure in one or more of the aqueous fluid channels, thereby to produce droplets of aqueous fluid in one or more outlet fluid channels.
2. The method of claim 1 , wherein the same constant pressure derives from pressure in a reservoir in fluid communication with each of the carrier fluid channels.
3. The method of claim 1 , wherein the same constant pressure is applied by a single pressure source to each of the carrier fluid channels.
4. The method of claim 3 , wherein the pressure source comprises carrier oil.
5. The method of claim 1 , wherein the droplets comprising a first aqueous fluid and the droplets comprising a second aqueous fluid are substantially uniform in size.
6. The method of claim 1 , wherein the droplets comprising a first aqueous fluid are a different size than the droplets comprising a second aqueous fluid.
7. The method of claim 5 or 6 , further comprising the step of detecting the size of the droplets in one or more of the aqueous fluid channels.
8. The method of claim 7 , further comprising the step of changing the pressure applied to the carrier fluid and/or the pressure applied to the one or more of the aqueous fluid channels based on the detecting step.
9. The method of claim 6 , further comprising the step of changing the pressure applied to a first of the aqueous fluid channels based on the detecting step, thereby to change the size of the droplets in the first aqueous fluid channel and not in other aqueous fluid channels.
10. The method of claim 1 , wherein pressure is not regulated in at least one of the aqueous carrier channels.
11. The method of claim 1 , wherein pressure is regulated in only one of the plurality of aqueous fluid channels.
12. The method of claim 1 , wherein the immiscible carrier fluid is an oil.
13. The method of claim 12 , wherein the oil comprises a surfactant.
14. The method of claim 13 , wherein the surfactant is a fluorosurfactant.
15. The method of claim 1 , wherein the aqueous fluid comprises nucleic acid and reagents for conducting an amplification reaction.
16. The method of claim 15 , wherein the amplification reaction is a PCR reaction.
17. The method of claim 1 , wherein said aqueous fluid channels comprise a plurality of microfluidic circuits, each of which comprises an independent aqueous fluid channel and wherein all members of said plurality of microfluidic circuits comprise a common immiscible carrier fluid channel.