Compositions, methods and uses for poxvirus elements in vaccine constructs against influenza virus subtypes or strains
Embodiments of the present invention generally disclose methods, compositions and uses for generating and expressing poxvirus constructs. In some embodiments, constructs may contain an influenza virus gene segment. In certain embodiments, methods generally relate to making and using compositions of constructs including, but not limited to, poxvirus vaccine compositions having two or more influenza gene segments. In other embodiments, vaccine compositions are reported of use in a subject.
1. A construct comprising:
a modified vaccinia virus construct encoding at least one vaccinia virus secretory signal sequence associated with a first influenza protein, and at least a second influenza protein;
wherein the at least one vaccinia virus secretory signal sequence comprises, at least one of a secretory signal sequence of C13L or B8R;
wherein the influenza proteins in the construct are capable of inducing an immune response in a subject.
2. The construct of claim 1 , wherein the first or second influenza protein comprises hemagglutinin (HA), neuraminidase (NA), nucleoprotein (NP), or matrix (M) or a combination thereof.
3. The construct of claim 1 , wherein the vaccinia virus secretory signal sequence is fused to the influenza protein.
4. The construct of claim 1 , further comprising one or more translational control sequences.
5. The construct of claim 1 , wherein the vaccinia virus is a modified vaccinia Ankara (MVA) virus.
6. The construct of claim 1 , wherein at least one of the influenza proteins comprises a naturally-occurring influenza protein with a naturally-occurring influenza secretory signal sequence.
7. The construct of claim 1 , wherein the influenza proteins are from different influenza subtypes or different influenza strains.
8. The construct of claim 1 , wherein at least one influenza protein is from an influenza A, B or C virus.
9. The construct of claim 1 , wherein the first influenza protein comprises the influenza protein nucleoprotein (NP); and the second influenza protein comprise the influenza protein hemagglutinin (HA).
10. A pharmaceutical composition comprising the construct of claim 1 ; and a pharmaceutically acceptable excipient.
11. The pharmaceutical composition of claim 10 , further comprising one or more additional poxvirus constructs having non-poxvirus antigens or fragments thereof.
12. A method for inducing an immune response to an influenza virus in a subject comprising:
administering the pharmaceutical composition of claim 10 to the subject in an amount sufficient to induce an immune response.
13. The method of claim 12 , wherein at least one of the influenza proteins comprises hemagglutinin (HA), neuraminidase (NA), nucleoprotein (NP), or matrix (M).
14. The method of claim 12 , wherein at least one influenza protein is obtained from an influenza A, B or C virus.
15. The method of claim 12 , further comprising administering a second composition comprising:
one or more poxvirus constructs prior to or at the same time as administering the pharmaceutical composition of claim 10 to the subject, wherein the second composition is also capable of inducing an immune response in the subject.
16. The method of claim 15 , wherein the pharmaceutical composition is administered to the subject 6 months or less prior to the second composition.
17. The method of claim 12 , wherein administration of the pharmaceutical composition comprises administering the composition intradermally, subcutaneously, intravenously, orally, intranasally, or by inhalation.
18. A vaccine kit comprising;
the pharmaceutical compositions of claim 10 ; and
at least one container.