Baffled single use bioreactor

1. A disposable bioprocessing apparatus intended for receiving a fluid in need of agitation and sparging using a gas, comprising:

a first sheet of film material having a first peripheral region;

a second sheet of film material having a second peripheral region and overlapping said first sheet of film material such that said first and second peripheral regions of said first and second sheets of film material are joined together so as to form a peripheral region of a bag and to define an interior space within said bag between said first and second sheets of film material;

at least two rows of staggered seals sealing said first sheet to said second sheet to create three baffled compartments, right, left and middle, within said bag, the at least two rows of staggered seals including a first row of spaced seals and a second row of spaced seals, the seals in the first row offset from the seals in the second row in a direction substantially perpendicular to a direction of at least one of the first row and the second row;

at least one sparging rod disposed in said middle compartment and connected to a source of sterile compressed gas;

a hard surface to support said bag;

at least one fluid port in said bag;

at least one gas exhaust port in said bag; and

at least one gas inlet port in said bag.

2. The apparatus according to claim 1 , wherein the hard surface comprises a rocking platform capable of tilting to such an angle as to cause movement of a fluid added to the apparatus from said right compartment to said left compartment passing through said middle compartment and vice versa.

3. The apparatus according to claim 1 , further comprising a movable element capable of applying pressure to the apparatus such that a fluid added to the apparatus moves from the left or right compartment to the other compartment passing through said middle compartment and vice versa.

4. The apparatus according to claim 1 , wherein said two rows of seals are set 1 to 5 inches apart.

5. The apparatus according to claim 1 , wherein said seals are set 0.2 to 2 inches apart.

6. The apparatus according to claim 1 , wherein said two rows of seals are generally located in the middle of said bag.

7. The apparatus according to claim 1 , wherein said seals are round or linear in shape or a combination of round and linear.

8. The apparatus according to claim 1 , wherein said seals in said two rows are staggered to create an indirect path of fluid moving through said middle compartment.

9. The apparatus according to claim 1 , further including a sensor for sensing a condition of said fluid in said bag.

10. The apparatus according to claim 1 , further including a means of heating or cooling said fluid in said bag.