Chloroplasts engineered to express pharmaceutical proteins
View Patent ↗Vaccines for conferring immunity in mammals to infective pathogens are provided, as well as vectors and methods for plastid transformation of plants to produce protective antigens and vaccines for oral delivery. The vaccines are operative by parenteral administration as well. The invention also extends to the transformed plants, plant parts, and seeds and progeny thereof. The invention is applicable to monocot and dicot plants.
1. A method of retarding the development of diabetes in a subject in need thereof comprising providing a composition comprising a Cholera toxin subunit B (CTB) proinsulin (Pins) fusion polypeptide and at least one chloroplast genome having a transgene inserted therein that encodes said fusion polypeptide and administering to said subject said composition; wherein said fusion polypeptide comprises pro-insulin consisting of an insulin A-chain, an insulin B-chain and an insulin C-peptide; and a furin cleavage site located between said CTB and proinsulin on the fusion polypeptide, said furin site increasing the efficiency of processing of proinsulin to mature insulin during receptor mediated delivery, said Cholera toxin subunit B proinsulin fusion polypeptide being effective to increase recruitment of IL-10 or IL-4 producing cells to the pancreas and induce tolerance in said subject.
2. The method of claim 1 , wherein said fusion polypeptide comprises a furin cleavage site located between said insulin A-chain and said insulin C-peptide.
3. The method of claim 1 , wherein said fusion polypeptide comprises a furin cleavage site located between said insulin B-chain and said insulin C-peptide.
4. The method of claim 1 , wherein said fusion polypeptide is effective to reduce expression of caspase-3 and apoptosis in pancreatic beta cells in said subject.
5. The method of claim 4 comprising determining caspase-3 levels following delivery of said fusion protein to said subject.