Regulatory region having increased expression and method of using same
An enhanced globulin-1 regulatory region is shown, a nucleotide sequence of which includes at least one additional copy of a region of the globulin-1 regulatory region which includes at least one transcriptional factor binding domain, combined with a transcription initiation site and translation start site. The promoter provides improved seed preferred, and particularly embryo preferred expression in plants. Methods of use are also shown in preferentially expressing a heterologous protein to the embryo tissue of a plant.
1. A DNA construct comprising an enhanced globulin-1 regulatory region comprising three copies of SEQ ID NO: 10 operably linked to a nucleic acid molecule.
2. A vector comprising the DNA construct of claim 1 .
3. A plant, plant part or plant cell comprising the DNA construct of claim 1 .
4. A method of increasing expression of a nucleic acid molecule in a plant, plant part or plant cell, the method comprising
introducing into said plant, plant part or cell a DNA construct, said DNA construct comprising an enhanced globulin-1 regulatory region operably linked to a nucleic acid molecule, said enhanced globulin-1 regulatory region comprising
three copies of SEQ ID NO: 10,
wherein said nucleic acid molecule is expressed at a higher level in a plant, plant part, or plant cell as compared to the expression of two copies of said nucleic acid molecule each copy operably linked to one copy of SEQ ID NO: 10.
5. The method of claim 4 , said method further comprising assaying said plant, plant part or plant cell for expression of said operably linked nucleic acid molecule and selecting the plants, plant parts or plant cells having said higher level of expression.