Apparatus and method for genetically transforming cells
View Patent ↗A fluid containing cells and free genetic material is acoustically coupled to a propulsion surface of a diaphragm. A blast-receiving surface of the diaphragm is acoustically coupled to an explosion chamber in which an explosive material is disposed. An ignition system ignites the explosive material in the explosion chamber to create a blast wave. The diaphragm transfers momentum from the blast wave to the fluid containing cells and free genetic material sufficient to cause the cells to take up the free genetic material.
1. A method for genetically transforming cells, comprising:
filling a container with a fluid, said fluid including said cells and free genetic material;
positioning a diaphragm such that a propulsion surface of said diaphragm contacts said fluid medium in said container, said diaphragm having a blast-receiving surface opposite said propulsion surface;
positioning a tube, said tube having a first open end and a second open end, such that said first open end is acoustically coupled to said blast-receiving surface of said diaphragm, said tube having an explosive material disposed therein; and
igniting said explosive material in said tube to create blast wave with a peak pressure that does not exceed 5 MPa, wherein said diaphragm transfers pressure from said blast wave to said fluid sufficient to cause said cells to take up said free genetic material.
2. The method of claim 1 , wherein said cells are bacterial cells.
3. The method of claim 2 , wherein said free genetic material comprises plasmid DNA.
4. The method of claim 1 , wherein igniting said explosive material in said tube to create a blast wave comprises:
applying electrical energy from a power supply to said explosive material in said tube.
5. The method of claim 4 , further comprising: electrically coupling said tube to said power supply by inserting at least one electrode into said second open end.
6. The method of claim 4 , wherein applying electrical energy from a power supply to said explosive material in said tube comprises:
stepping up a first voltage from said power supply to a second voltage;
charging a capacitor with said second voltage; and
discharging said capacitor to create a spark in said tube.
7. The method of claim 1 , wherein said explosive material comprises conventional HMX.
8. The method of claim 7 , wherein said explosive material is a mixture of conventional HMX with aluminium powder.
9. The method of claim 1 , wherein said explosive is coated at about 18 mg/m on the interior surface of said tube.