Mechanical holder for surface analysis
A mechanical holder that provides for a confined sampling region for extraction and removal of chemical substances contained in a dried blood spot or other spot of sample is described herein.
1. A system, comprising:
a lower portion configured to house an sample card;
an upper portion mechanically connected to the lower portion, the upper portion including an array of fittings configured such that when the upper portion and the lower portion are in a closed position, a substantially liquid tight seal is formed around multiple, different portions of the sample card.
2. The system of claim 1 , wherein the liquid tight seal is configured to confine at least 95% of the extraction solvent within the walls of the fitting.
3. The system of claim 1 , wherein the sample card that includes a dried sample.
4. The system of claim 1 , wherein the fittings are configured to receive a solvent delivery device and include a rigid lower perimeter edge.
5. The system of claim 1 , wherein the fittings are configured to receive a solvent delivery device capable of delivering an extraction solvent for extracting chemicals from the sample substrate material.
6. The system of claim 1 , wherein the fittings are configured to receive a pipette tip.
7. The system of claim 1 , wherein the fitting comprises PEEK, polymer, brass or stainless steel with a rigid lower circular perimeter edge capable of making a leak-tight seal when clamped down upon the sample card without cutting a disk from the sample card.
8. The system of claim 1 , further comprising a robotic device configured to extract analytes from the sample card to form a solution from a surface of the sample card using a solvent delivery device which can then be withdrawn from the surface and delivered to a mass spectrometer.
9. The system of claim 1 wherein the fitting is configured such that extraction solvent introduced into the fitting is substantially confined within the walls of the fitting.
10. The system of claim 1 , further comprising a locking mechanism configured to compress the sample card.
11. The system of claim 10 , wherein the locking mechanism is configured to depress the sample card by between about 0.015 inch to about 0.030 inch to form the substantially liquid tight seal.
12. The system of claim 10 , wherein the locking mechanism comprises one or more screws.
13. The system of claim 1 , wherein the fitting comprises PEEK with a rigid lower circular perimeter edge capable of making a leak-tight seal when clamped down upon the sample card without cutting a disk from the sample card.
14. The system of claim 1 , wherein the fitting comprises brass or stainless steel with a rigid lower circular perimeter edge capable of making a leak-tight seal when clamped down upon the sample card without cutting a disk from the sample card.
15. The system of claim 1 , wherein the array of fittings comprises an array of 96 fittings.
16. The system of claim 1 , wherein the liquid tight seal is configured to confine at least 98% of the extraction solvent within the walls of the fitting.
17. The system of claim 1 , wherein the array of fittings comprises at least four fittings.
18. The system of claim 1 , wherein the sample card comprises a porous surface.
19. The system of claim 1 , wherein the fittings comprise cylindrical fittings with a diameter of 0.1 mm to 1 mm.
20. The system of claim 1 , wherein the fittings comprise cylindrical fittings with a diameter of 2 mm to 5 mm.