Analyte concentration detection devices and methods
Arrangements for the detection of the presence and/or concentration of an analyte in a sample of bodily fluid include diffuse transmission, diffuse reflection and edge or waveguide illumination arrangements. A vertical flow assay arrangement and/or technique is also disclosed, and includes a detection component that can be in the form of an array of optical detection elements. A number of assay pad constructions are described which may include at least one or more of the following components: a prefilter component, a reflective component, a membrane component, a reagent component, a mesh component, and a component to prevent lateral spreading.
1. A device for conducting an assay to determine the concentration of an analyte in a sample of bodily fluid, the device comprising:
a microneedle configured to collect the sample and comprising a lumen therethrough;
an elongate sample collection channel transverse to the lumen of the microneedle, wherein the sample collection channel comprises a proximal end and a distal end, and wherein the proximal end is in communication with the lumen of the microneedle;
an assay pad comprising a reagent that produces a reaction when exposed to the analyte, wherein the assay pad is in communication with the sample collection channel along a length between the proximal and distal ends of the sample collection channel; and
a linear array of detectors configured to scan a line across the assay pad to detect the reaction.
2. The device of claim 1 wherein the linear array of detectors comprises a sensor, the sensor comprising a CMOS sensor, a CCD sensor, or a photodiode.
3. The device of claim 1 , wherein the analyte comprises glucose.
4. The device of claim 1 , wherein the linear array of detectors comprises a linear array of CMOS sensors.
5. The device of claim 1 , further comprising a light source adapted to provide light incident upon the assay pad.
6. The device of claim 1 , further comprising a lens disposed between the linear array of detectors and the assay pad.
7. The device of claim 6 , wherein the lens is a refractive lens.
8. The device of claim 6 , wherein the lens is a diffractive lens.