Method and apparatus for collecting biological materials
View Patent ↗A method and apparatus can separate and concentrate a selected component from a multi-component material. The multi-component material may include a whole sample such as adipose tissue, whole blood, or the like. The apparatus generally includes a moveable piston positioned within a separation container and a withdrawal tube that is operable to interact with a distal end of the collection container past the piston. Material can be withdrawn through the withdrawal tube.
1. A method of separating a component from a selected material, comprising:
obtaining the selected material having multiple components;
providing a separation system including a tube having a top wall and a bottom wall, a piston within the tube, an input port defined through the top wall, an extraction port defined through the top wall, a hollow member extending from the extraction port at least to the piston;
positioning the selected material into the separation system through the input port with the top wall fixed to the tube and between the top wall and the piston; centrifuging the separation system containing the selected material thereby causing and allowing the piston to move towards the top wall during centrifugation; and
extracting the component from the selected material through the hollow tube from past an entire structure of the piston and between an exterior wall of the piston and the bottom wall of the tube.
2. The method of claim 1 , wherein positioning the selected material into the separation system includes positioning the selected material substantially only between the top wall and a first top side of the piston.
3. The method of claim 2 , wherein centrifuging the separation system and allowing the piston to move towards the top wall includes:
separating the selected material into separate components; and
allowing the piston to move relative to a first of the components wherein at least a portion of the first component is positioned between the bottom wall of the tube and the exterior wall of the piston that includes a second external bottom side of the piston.
4. The method of claim 3 , wherein extracting the component includes withdrawing at least a portion of the first component from between the bottom wall of the tube and the second external bottom side of the piston through the hollow member.
5. The method of claim 1 , further comprising:
providing the hollow member substantially rigidly within the tube;
providing a stop member extending from the hollow member; and
stopping the piston from moving towards the top wall during centrifugation with the stop member.
6. The method of claim 1 , further comprising:
coupling a separate injection port extender to the input port before positioning a selected material into the separation system; and
coupling a collection device that collected the selected material to the injection port extender.
7. The method of claim 1 , further comprising:
coupling a collection device to the input port of the separation system to position the selected material into the separation system.
8. The method of claim 4 , wherein withdrawing at least a portion of the first component from between the bottom wall of the tube and the second external bottom side of the piston through the hollow member further includes withdrawing a portion of the first component through first top side and the second external bottom side of the piston.
9. A method of separating a component from a selected material, comprising:
obtaining the selected material with a collection device;
engaging the collection device to an input port of a separation system having a container that includes a top wall and a bottom wall, a piston within the container, and a withdrawal tube extending to the piston;
delivering the selected material through the input port of the separation system with the collection device;
removing the collection device from the separation system;
centrifuging the separation system containing the selected material; and
extracting the selected component from the selected material through the withdrawal tube within the collection device from past an entire structure of the piston and between a bottom external terminal wall of the piston and the bottom wall of the container.
10. A method of separating a component from a selected material, comprising:
obtaining the selected material with a collection device;
engaging the collection device to an input port of a separation system having a container that includes a top wall and a bottom wall, a piston within the container, and a withdrawal tube extending to the piston;
delivering the selected material through the input port of the separation system with the collection device;
removing the collection device from the separation system;
centrifuging the separation system containing the selected material;
extracting the component of the selected material from between the piston and the bottom wall of the container;
allowing the piston to move toward the top wall during centrifugation; and
allowing the piston to engage a stop member extending from the withdrawal tube to stop the piston from moving toward the top wall.
11. The method of claim 9 , wherein extracting the component includes withdrawing at least a portion of the component from between the bottom wall of the container and the bottom external terminal wall of the piston through a withdrawal port in communication with the withdrawal tube.
12. The method of claim 11 , further comprising:
withdrawing at least a portion of the component through the piston and the withdrawal tube upon coupling a withdrawal container to the withdrawal port.
13. The method of claim 9 , wherein delivering the selected material further includes positioning the selected material substantially only between the top wall and a first side of the piston.
14. The method of claim 10 , further comprising:
wherein allowing the piston to move toward the top wall during centrifugation includes allowing the piston to move along the withdrawal tube during centrifugation until the piston engages the stop member extending from the withdrawal tube.
15. A method of separating a component from a selected material, comprising:
collecting the selected material with a collection device;
coupling the collection device to an input port of a separation system having a container that includes a top wall, a bottom wall and a piston movable on a withdrawal tube within the container;
delivering the selected material through the input port of the separation system with the collection device;
removing the collection device from the separation system;
positioning the separation system in a centrifuge to apply a force to the selected material in the separation system to allow the piston to move toward the top wall of the container during centrifugation and sequester the component between the piston and the bottom wall of the container;
withdrawing the component from the separation system through the withdrawal tube; and
allowing the piston to move toward the top wall during centrifugation until the piston engages a stop member extending from the withdrawal tube.
16. The method of claim 15 , wherein withdrawing the component further includes withdrawing stromal cells from the separation system.
17. The method of claim 15 , wherein sequestering the component from the selected material further includes holding the piston at a selected position relative to the withdrawal tube and holding the piston fixed relative to the component of the selected material.
18. The method of claim 15 , wherein withdrawing the component of the selected material further includes coupling a withdrawal device to a withdrawal port of the container and withdrawing the component through the piston and the withdrawal tube.
19. The method of claim 15 , further comprising:
coupling an injection port extender to the input port before coupling the collection device to the input port of the separation system.