Passive methods for anti-microbial biological meshes
View Patent ↗Tissue matrices having anti-microbial properties are provided. In certain embodiments, the tissue matrices include cationic anti-microbial agents that form a stable bond with the tissue matrices without adversely affecting the biologic properties of the tissue matrices.
1. A method of preparing a modified tissue matrix comprising:
selecting a collagen-based acellular tissue matrix;
contacting the acellular tissue matrix with an aqueous solution containing a cationic anti-microbial agent, wherein the agent stably binds to the acellular tissue matrix by passive adsorption; and
exposing the acellular tissue matrix to E-beam radiation.
2. The method of claim 1 , wherein the aqueous solution is non-denaturing.
3. The method of claim 1 , wherein the acellular tissue at matrix comprises an acellular porcine dermal matrix.
4. The method of claim 1 , wherein the anti-microbial compound comprises at least one of benzethonium chloride, chlorhexidine, benzalkonium chloride, silver, poly-hexa-methylene biguanide hydrochloride, cationic peptides, chitosan, or quaternary ammonium compounds.
5. The method of claim 1 , wherein the acellular tissue matrix is not cross-linked or denatured during binding of the anti-microbial agent.
6. The method of claim 1 , wherein the anti-microbial agent is not covalently modified by binding to the acellular tissue matrix.
7. The method of claim 1 , wherein the modified tissue matrix retains anti-microbial properties for at least two weeks after binding of the anti-microbial agent.
8. The method of claim 1 , wherein the antimicrobial agent remains bound to the modified tissue matrix when placed in a non-denaturing aqueous solution.
9. The method of claim 1 , wherein the antimicrobial agent remains bound to the modified tissue matrix when placed in PBS.
10. The method of claim 1 , wherein the bound anti-microbial agent retains greater than 95% of its activity when the modified tissue matrix is placed in PBS for two weeks at room temperature.
11. The method of claim 1 , wherein the bound anti-microbial agent retains greater than 75% of its activity when the modified tissue matrix is placed in PBS for two weeks at room temperature.
12. The method of claim 1 , wherein contacting the acellular tissue matrix with an aqueous solution containing a cationic anti-microbial agent comprises contacting the tissue matrix with an aqueous solution for four hours at room temperature.