VINCRISTINE IMMUNOASSAY
Novel conjugates and immunogens derived from vincristine and antibodies generated by these immunogens are useful in immunoassays for the quantification and monitoring of vincristine in biological fluids.
1 . An antibody which bonds to selectively binds to vincristine and does not substantiate cross react by binding with the pharmaceutically inactive vincristine metabolite, M1.
2 . The antibody of claim 1 wherein said selectively reactive antibody, selectively binds to vincristine without any substantial cross reactivity to bind with vinblastine, vindesine, or vinorelbine, and with the pharmaceutically inactive vincristine metabolite, M1.
3 . The antibody of claim i wherein said antibody is generated from an immunogen comprising an immunogenic carrier containing a polyamine polymer conjugated with a ligand of the formula:
wherein B is
Y is an organic spacing group;
X is a terminal functional group capable of binding to an immunogenic carrier containing a polyamine polymer; and
p is an integer from 0 to 1
4 . The antibody of claim 14 , wherein said antibody is derived from mice, sheep, rabbits or rats.
5 . The antibody of claim 3 , wherein said antibody is a monoclonal antibody.
6 . A compound of the formula:
wherein B is
Y is an organic spacing group;
X is a terminal functional group capable of binding to a carrier; and
p is an integer from 0 to 1.
7 . The compound of claim 6 , wherein p is 0.
8 . The compound of claim 7 , wherein X is
wherein R 3 is hydrogen, a halide, a hydroxyl, or taken together with its attached oxygen atom forms a reactive ester and R 4 is oxygen or sulfur.
9 . The compound of claim 8 wherein X is
10 . The compound of claim 8 wherein X is
11 . The compound of claim 8 , wherein X is
and R 3 is hydroxyl.
12 . The compound of claim 8 , wherein X is
and R 3 forms a reactive ester.
13 . The compound of claim 12 , wherein the ester formed is a lower alkyl ester, imidoester or amidoester.
14 . The compound of claim 6 , wherein p is 1.
15 . The compound of claim 14 , wherein X is
wherein R 3 is hydrogen, a halide, a hydroxyl, or taken together with its attached oxygen atom forms a reactive ester and R 4 is oxygen or sulfur.
16 . The compound of claim 15 , wherein Y is alkylene containing from 1 to 10 carbon atoms,
wherein n and o are integers from 0 to 6, and m is an integer from 1 to 6.
17 . The compound of claim 16 , wherein Y is lower alkylene containing from 1 to 6 carbon atoms.
18 . The compound of claim 17 , wherein X is
and R 3 is as above.
19 . The compound of claim 16 , wherein Y is
wherein m and o are as above.
20 . The compound of claim 19 , wherein X is
and R 3 is as above.
21 . A conjugate comprising a carrier conjugated with a ligand of the formula:
wherein B is
Y is an organic spacing group;
X is a functional linking group capable of linking to a carrier; and
p is an integer from 0 to 1.
22 . The conjugate of claim 21 , wherein the carrier contains a polyamine polymer.
23 . The conjugate of claim 21 , wherein p is 0.
24 . The conjugate of claim 23 , wherein X is
wherein R 3 is hydrogen, a halide, a hydroxyl, or taken together with its attached oxygen atom forms a reactive ester and R 4 is oxygen or sulfur.
25 . The conjugate of claim 21 , wherein p is 1.
26 . The conjugate of claim 25 , wherein Y is alkylene containing from 1 to 10 carbon atoms,
wherein n and o are integers from 0 to 6, and m is an integer from 1 to 6.
27 . The conjugate of claim 26 , wherein X is
wherein R 3 is hydrogen, a halide, a hydroxyl, or taken together with its attached oxygen atom forms a reactive ester and R 4 is oxygen or sulfur.
28 . The conjugate of claim 21 , wherein said polyamine polymer is an immunogenic polymer.
29 . The conjugate of claim 26 wherein Y is an alkylene containing from 1 to 6 carbon atoms.
30 . The conjugate of claim 29 , wherein X is
and R 3 is as above.
31 . The conjugate of claim 26 , wherein Y is
where m and o are as above.
32 . The conjugate of claim 31 , wherein X is
and R 3 is as above.