Methods and devices for preserving tissues
View Patent ↗Methods and apparatus for preserving detached tissues, especially digits and limbs, which are detached as a result of traumatic amputation. By using the methods and apparatus, detached tissues can be preserved for greater lengths of time and are ultimately in a better condition for replantation surgery.
1. A portable apparatus for preserving severed tissue, comprising:
a tissue chamber; and
a lid assembly configured to mate with the tissue chamber, the lid assembly comprising:
a pumping chamber having a semi-permeable membrane disposed at an inclined angle with respect to horizontal when the lid assembly and tissue chamber are mated and placed on a horizontal surface, the semi-permeable membrane being configured to push against a preservation fluid and cause the preservation fluid to perfuse a severed tissue in the tissue chamber with oxygenated preservation fluid at a pressure greater than atmospheric pressure;
a fill port defining a lumen having a first opening and a second opening, wherein the first opening opens to the exterior of the lid assembly and the second opening opens to the tissue chamber; and
a purge port connecting the exterior of the lid assembly to the pumping chamber, thus allowing the apparatus to self-purge a fluid from the tissue chamber when the tissue chamber and lid assembly are mated, and the tissue chamber is filled with a liquid via the fill port.
2. The apparatus of claim 1 , wherein the membrane is disposed within the lid assembly at an angle such that when the lid assembly and the tissue chamber are mated and placed on a horizontal surface the membrane can direct a rising fluid toward the purge port.
3. The apparatus of claim 2 , wherein the purge port is configured to permit the rising fluid to flow to an external area.
4. The apparatus of claim 2 , wherein the membrane is inclined at an angle of approximately 1° -10° with respect to a line perpendicular to gravity when the apparatus is used to transport severed tissue.
5. The apparatus of claim 2 , wherein the membrane flexes in response to a pressure increase in the first portion of the pumping chamber, thereby exerting a pressure against the preservation fluid.
6. The apparatus of claim 1 , wherein the lid assembly is removably coupleable to the tissue chamber.
7. The apparatus of claim 2 , wherein the lid assembly comprises a pneumatic system configured to generate a positive pressure in the first portion of the pumping chamber.
8. The apparatus of claim 1 , wherein the fluid is preservation fluid.
9. The apparatus of claim 1 , wherein the fluid is a gas.
10. The apparatus of claim 1 , wherein the apparatus is capable of self-purging a fluid while perfusing the severed tissue.
11. The apparatus of claim 1 , further comprising a basket or sling to receive the severed tissue.
12. The apparatus of claim 1 , wherein the apparatus is capable of regulating the pressure of the preservation fluid by self-purging the preservation fluid.
13. The apparatus of claim 1 , wherein the purged fluid is directed to an external area.
14. The apparatus of claim 2 , wherein the rising fluid is a gas.