Methods and compositions for diagnosis and prognosis of renal injury and renal failure
View Patent ↗The present invention relates to methods and compositions for monitoring, diagnosis, prognosis, and determination of treatment regimens in subjects suffering from or suspected of having a renal injury. In particular, the invention relates to using a plurality of assays configured to detect a kidney injury marker as diagnostic and prognostic biomarkers in renal injuries.
1. A method for evaluating the likelihood of future nonoccurrence of acute renal failure renal in a critically ill subject diagnosed with acute renal injury, comprising:
obtaining a plasma or urine sample from a the subject,
introducing the plasma or urine sample obtained from the subject into an assay instrument for performing a first immunoassay to determine the concentration of antileukoproteinase and a second immunoassay to determine the concentration of thrombomodulin wherein the first immunoassay comprises: contacting the plasma or urine sample with a first antibody specifically binding antileukoproteinase to obtain a first assay result value indicative of the concentration value of Antileukoproteinase,
wherein the second immunoassay comprises: contacting the plasma or urine sample with a second antibody specifically binding thrombomodulin to generate a second assay result value indicative of the concentration value of Thrombomodulin,
obtaining a composite value comprising the ratio of concentration of Antileukoproteinase to the concentration value of thrombomodulin,
correlating the composite value to a likelihood of occurrence or nonoccurrence of continuing acute renal failure within 72 hours of the time at which the plasma or urine sample is obtained from the subject,
wherein the composite value that is lower as compared to a threshold is indicative of a likelihood of nonoccurrence of continuing acute renal failure within 72 hours of the time at which the plasma or urine sample is obtained from the subject; and
wherein the composite value that is higher as compared to the threshold is indicative of a likelihood of occurrence of continuing acute renal failure within 72 hours of the time at which the plasma or urine sample is obtained from the subject; and
wherein the threshold is a composite result value obtained by performing the first and second immunoassays in plasma or urine sample from a predetermined subpopulation of individuals comprising critically ill subjects with a known occurrence of continuing acute renal failure within 72 hours of the time at which the plasma or urine sample is obtained.
2. A method according to claim 1 , wherein the correlating step comprises correlating the composite value to a likelihood of occurrence or nonoccurrence of continuing acute renal failure within 48 hours of the time at which the plasma or urine sample is obtained from the subject,
wherein the composite value that is lower as compared to a threshold is indicative of a likelihood of nonoccurrence of continuing acute renal failure within 48 hours of the time at which the plasma or urine sample is obtained from the subject; and
wherein the composite value that is higher as compared to the threshold is indicative of a likelihood of occurrence of continuing acute renal failure within 48 hours of the time at which the plasma or urine sample is obtained from the subject; and
wherein the threshold is a composite result obtained by performing the first and second immunoassays in plasma or urine sample from a predetermined subpopulation of individuals comprising critically ill subjects with a known occurrence of continuing acute renal failure within 48 hours of the time at which the plasma or urine sample is obtained.
3. A method according to claim 1 , wherein the correlating step comprises correlating the composite value to a likelihood of occurrence or nonoccurrence of continuing acute renal failure within 24 hours of the time at which the plasma or urine sample is obtained from the subject,
wherein the composite value that is lower as compared to a threshold is indicative of a likelihood of nonoccurrence of continuing acute renal failure within 24 hours of the time at which the plasma or urine sample is obtained from the subject; and
wherein the composite value that is higher as compared to the threshold is indicative of a likelihood of occurrence of continuing acute renal failure within 24 hours of the time at which the plasma or urine sample is obtained from the subject; and
wherein the threshold is a composite result obtained by performing the first and second immunoassays in plasma or urine sample from a predetermined subpopulation of individuals comprising critically ill subjects with a known occurrence of continuing acute renal failure within 24 hours of the time at which the plasma or urine sample is obtained.
4. A method according to claim 1 , wherein the subject is in RIFLE stage I or F.