Derivation of hematopoietic cells from adult mesenchymal stem cells

1. A process of generating a population of cells for hematopoietic cell transplantation comprising:

collecting a volume of adipose tissue from a mammal and cleaning non-tissue bound circulating cells from the tissue;

isolating stromal vascular fraction (SVF) cells from the cleaned adipose tissue, said SVF cells isolated by enzymatically digesting the cleaned adipose tissue and removing lipid containing cells;

expanding the SVF cells in vitro to generate a population of Adipose-derived Stem Cells (ADSC) that are CD34−;

culturing the CD34− ADSC in a hematopoietic differentiation media that promotes differentiation to a hematopoietic lineage comprising one or more differentiation factors selected from the group consisting of IL-1β, IL-3, IL-4, IL-5, G-CSF, MCSF and GM-CSF; and

collecting the cultured cells for transplantation.

2. The process of claim 1 , further comprising exposing the SVF cells to plastic adherence wherein nonadherent cells are discarded prior to expanding the SVF cells in vitro to generate the population of CD34− ADSC.

3. The process of claim 1 , wherein negative selection is applied to insure essentially complete removal of any residual CD34+ cells from the CD34− ADSC prior to culture in the hematopoietic differentiation media.

4. The process of claim 1 , wherein the hematopoietic differentiation media promotes differentiation into neutrophils and includes G-CSF.

5. A process of generating a population of cells for hematopoietic cell transplantation comprising:

collecting a volume of adipose tissue from a mammal and cleaning non-tissue bound circulating cells from the tissue;

isolating stromal vascular fraction (SVF) cells from the cleaned adipose tissue, said SVF cells isolated by enzymatically digesting the cleaned adipose tissue and removing lipid containing cells;

expanding the SVF cells in vitro to generate a population of Adipose-derived Stem Cells (ADSC) that are CD34−;

culturing the CD34− ADSC in a hematopoietic differentiation media that promotes differentiation into macrophages and includes IL-1β, IL-3, and M-CSF; and

collecting the cultured cells for transplantation.

6. The process of claim 5 , wherein the hematopoietic differentiation media further comprises 1-monothioglycerol (1-MTG).

7. A process of generating a population of cells for hematopoietic cell transplantation comprising:

collecting a volume of adipose tissue from a mammal and cleaning non-tissue bound circulating cells from the tissue;

isolating stromal vascular fraction (SVF) cells from the cleaned adipose tissue, said SVF cells isolated by enzymatically digesting the cleaned adipose tissue and removing lipid containing cells;

expanding the SVF cells in vitro to generate a population of Adipose-derived Stem Cells (ADSC) that are CD34−;

culturing the CD34− ADSC in a hematopoietic differentiation media that promotes differentiation into eosinophils and includes IL-3 and IL-5; and

collecting the cultured cells for transplantation.

8. A process of generating a population of cells for hematopoietic cell transplantation comprising:

collecting a volume of adipose tissue from a mammal and cleaning non-tissue bound circulating cells from the tissue;

isolating stromal vascular fraction (SVF) cells from the cleaned adipose tissue, said SVF cells isolated by enzymatically digesting the cleaned adipose tissue and removing lipid containing cells;

expanding the SVF cells in vitro to generate a population of Adipose-derived Stem Cells (ADSC) that are CD34−;

culturing the CD34− ADSC in a hematopoietic differentiation media that promotes differentiation into dendritic cells and includes GM-CSF and IL-4; and

collecting the cultured cells for transplantation.

9. A method of autologous stem cell transplantation comprising:

preparing a population of autologous stem cells from a patient by a procedure comprising the steps of:

removing a portion of adipose tissue from a patient and thoroughly washing the portion to remove non-tissue bound circulating cells prior to isolating a stromal vascular fraction (SVF) of cells from the washed adipose tissue;

expanding the SVF cells in vitro to generate a population of Adipose-derived Stem Cells (ADSC) that are CD34−;

culturing the CD34− ADSC in a differentiation media that comprises at least one differentiation factor selected from the group consisting of IL-1β, IL-3, IL-4, IL-5, G-CSF, MCSF, and GM-CSF;

collecting and storing the cultured cells until transplantation;

conditioning the patient by chemotherapy and/or radiation to destroy abnormal stem cells and blood cells;

isolating the patient for an extended post-conditioning period; and

transplanting the cultured cells into the patient.

10. The method of claim 9 , further comprising a step of exposing the SVF cells to plastic adherence wherein nonadherent cells are discarded prior to expanding the SVF cells in vitro to generate the population of CD34− ADSC .

11. The method of claim 9 , wherein negative selection is applied to insure essentially complete removal of any residual CD34+ cells from the CD34− ADSC prior to culture in the differentiation media.

12. The method of claim 9 , further comprising:

isolating a quantity of cancer-free autologous or allogeneic bone marrow trainer cells; and

co-culturing the bone marrow trainer cells with the SVF cells.

13. The method of claim 12 , wherein the cancer-free bone marrow trainer cells are mixed population of nucleated cells representing a respective distribution of hematopoietic lineages in the bone marrow.

14. The method of claim 12 , wherein the cancer-free bone marrow trainer cells are purified into subsets of cells and the subsets are co-cultured with the SVF.

15. The method of claim 12 , further comprising removing CD34+ cells from the SVF cells prior to co-culture.

16. The method of claim 12 , wherein the cancer-free bone marrow trainer cells have been treated by removal of CD34+ cells prior to co-culture.

17. The method of claim 12 , wherein the cancer-free bone marrow trainer cells have been treated by lethally irradiating the bone marrow trainer cells to render them cancer free prior to the co-culture.