Detection of synthetic cannabinoids
The invention describes methods and kits for detecting and determining current and future synthetic cannabinoids from the CP family. Unique antibodies derived from novel immunogens enable said methods and kits.
1. An antibody that binds to (±)-CP-47,497 (C 7 homologue) and (±)-CP-47,497 (C 8 homologue),
wherein the antibody has a cross-reactivity presented as B/B 0 of ≦50% against (±)-CP-47,497 (C 7 homologue) at a concentration of 100 ng/ml, and a cross-reactivity presented as B/B 0 of ≦50% against (±)-CP-47,497 (C 8 homologue) at a concentration of 100 ng/ml, and
wherein the antibody has a cross-reactivity presented as B/B 0 of greater than 80% against each one of the cross-reactants selected from the group consisting of (±)-CP-47,497 (C 7 para quinone analogue), (±)-CP-55,940, (−)-CP 55,940, (+)-CP 55, 940, HU-210, HU-211 (dexanabinol), HU-308, delta9-THC, and (−)-11-nor-9-carboxy-delta9-THC, at a cross-reactant concentration of 100 ng/ml,
as determined using (±)-CP-47,497 (C 8 homologue) as a standard and Tracer 1,
wherein Tracer 1 has the following structural formula:
wherein HRP is Horse Radish Peroxidase,
wherein B 0 is absorbance at 450 nm at zero ng/ml standard concentration, and
wherein B is absorbance at 450 nm at predetermined variable standard concentrations.
2. An antibody raised against an immunogen of the following structure:
wherein the accm is an antigenicity conferring carrier material;
wherein the crosslinker is a functionalised linking group joining the accm to the remainder of the molecule, the crosslinker forming a double bond to the cyclohexyl ring;
wherein m is 3;
wherein the antibody has a cross-reactivity presented as B/B 0 of ≦50% against (±) CP-47,497 (C 7 homologue) at a concentration of 100 ng/ml and a cross-reactivity presented as B/B 0 of ≦50% against (±)-CP-47,497 (C 8 homologue) at a concentration of 100 ng/ml, and
wherein the antibody has a cross-reactivity presented as B/B 0 of greater than 80% against each one of the cross-reactants selected from the group consisting of (±)-CP-47,497 (C 7 para quinone analogue), (±)-CP-55,940, (−)-CP 55,940, (+)-CP 55, 940, HU-210, HU-211 (dexanabinol), HU-308, delta9-THC, and (−)-11-nor-9-carboxy-delta9-THC, at a cross-reactant concentration of 100 ng/ml,
wherein the B/B 0 is measured using (±)-CP-47,497 (C 8 homologue) as a standard and Tracer 1 as a tracer, of the following structural formula:
wherein B 0 is absorbance at 450 nm at zero ng/ml standard concentration, and
wherein B is absorbance at 450 nm at predetermined variable standard concentrations.